Directional Microchannel Cellulose Bioreactor And Its Continuous Transformation Of Resveratrol Glycosides In Mulberry Trees

Mulberry has thousands of years of history in our country,and as an important food source for silkworms,they play an important role in the sericulture resources resources.As a kind of medicinal and edible plant,phenolic substances in mulberry have been widely used in many fields such as medical treatment and food because of their high content and high activity.However,the active phenolic components of mulberry were mainly glycosides with low bioavailability.At present,it has been proved that glycoside phenols can only exert their true pharmacological activity after removing the glycoside group and transforming into aglycone structure.Therefore,in this paper,resveratrol glycosides in active components of mulberry were taken as the research object.Inspired by the directional channel structure of natural wood,a continuous fluid biocatalytic system was constructed with xylem to explore the feasibility of converting the active component of glycosides into aglycones(resveratrol).Secondly,the bioreactor was optimized,and cellulose was used to artificially prepare directional channel structure to test the catalytic performance of the reactor and explore the influence of mass transfer inside the reactor on the catalytic performance.Finally,a cellulose dual-scale directional microchannel reactor was constructed to regulate the internal structure of the reactor,explore the impact of internal channel structure on mass transfer,heat transfer,and catalytic performance,and further optimize the catalytic performance of the reactor to maximize the conversion efficiency of aglycone active components.The specific research results are as follows:1.Using natural basswood as the substrate,the internal channels and pores of the wood are completely exposed through delignification and DMAc/Li Cl treatment.The natural directional channel and porous inner wall structure were used to immobilizeβ-glucosidase to prepare a bioreactor to catalyze the transformation of resveratrol glycosides in a continuous fluid catalytic system.After a catalytic reaction at p H 6 and temperature of 60 ℃ for 5 hours,the conversion efficiency reached 98.15%.The reactor has good reusability and storage stability.It is confirmed that the feasibility of using xylem as a substrate to prepare a bioreactor for converting active components of mulberry glycosides.2.Using cotton linter fiber as raw material,the directional channels and porous inner wall structure of xylem were prepared manually and rapidly by freeze casting.The obtained materials were modified with Ni O nanoparticles to bind the engineeriing enzyme protein(β-glucosidase with histidine label).The prepared bioreactor can achieve a conversion rate of 98.69% of resveratrol within 3 h,which is higher than other reactor reports,and the reactor also has good stability.It has found that the directional channels on the porous inner wall not only maintain the low pressure and rapid flow of fluid inside the reactor,but also generate "micro disturbances" during the fluid movement process,increasing the contact probability between the substrate and the catalyst,and strengthening the reaction mass transfer process3.Using different metal alloy materials as freezing substrates to regulate the internal channel structure of cellulose materials,a dual-scale directional microchannel reactor is constructed.The influence of channel structure on catalytic performance of reactor was investigated.After covalent fixation β-glucosidase,a high conversion rate of 98.84% can be achieved,confirming the enormous potential of dual scale microchannel structures in biocatalysis.Even at a high flow rate of 20 m L/h,the reactor can maintain a conversion rate of over 80% at a low pressure of 0.20 k Pa.The dual-scale directional microchannel gives the reactor faster material transport speed and larger transport capacity,makes the fluid have faster diffusion rate and more intense "micro-perturbation" in the reactor,accelerates the mass transfer state of the whole reaction,and further improves its catalytic performance.This structure provides a new idea for the construction of fluid biocataltic reactor.The various directional microchannel reactors constructed in this article achieves high efficiency conversion of resveratrol(The conversion rate was more than 98%)and has good stability and reusability(The activity decreased only by about 10% after storage for more than 15 days).Compared with most current reactors,this reactor has superior catalytic performance and huge application potential.It provides a more convenient and green way for the transformation of active components of mulberry aglycones,and also provides a new strategy for promoting the application of mulberry resources in the field of nutrition and health care.