| The purpose of this study was to compare the differences of serum biochemistry and liver function between IUGR newborn piglets and normal newborn piglets.On this basis,IUGR piglets were perfused with an appropriate amount of ser solution to explore the effects of ser on weaning weight,serum biochemistry and liver pathological changes,oxidative stress,amino acid and energy metabolism of IUGR piglets.The experiment was divided into two parts.In Experiment 1,IUGR newborn piglets and normal newborn piglets were taken as the experimental objects.The serum and liver related indexes of IUGR newborn piglets and normal newborn piglets were measured by slaughter sampling,and the differences of their birth weight and liver function were compared;In Experiment 2,1-day-old IUGR newborn piglets were perfused with 0.8% ser solution,and 1-day-old normal newborn piglets were used as control and perfused with normal saline.The experimental period was28 days to explore whether IUGR piglets could reach the level of normal piglets through catch-up growth.Experiment1:six litters of ternary hybrid newborn piglets [Duroc×(Landrance×Yorkshire)] meeting the selection criteria of normal birth weight and intrauterine growth retardation were selected.According to the selection method of one normal piglet and one IUGR male piglet in each litter,six normal piglets and six IUGR piglets were selected,and slaughtered,sampled and analyzed after blood collection and weighing.The results showed that:(1)the body weight of IUGR newborn piglets was significantly lower than that of normal piglets(P<0.01);(2)There were congestion,edema and inflammation in the liver of IUGR newborn piglets,and the liver index was significantly higher than that of normal newborn piglets(P<0.05);(3)The serum ALT of IUGR newborn piglets was significantly higher than that of normal newborn piglets(P<0.01),and AST was significantly lower than that of normal newborn piglets(P<0.01);(4)The serum free Gly and val of IUGR newborn piglets were significantly higher than those of normal piglets(P<0.05),the free THR,Ile and Leu were significantly higher than those of normal piglets(P<0.01),and the free ser was significantly lower than that of normal piglets(P<0.05);(5)A total of 66 differential metabolites(23 positive ion differential metabolites and 43 negative ion differential metabolites)were screened from the serum samples of IUGR newborn piglets and normal newborn piglets.The differential metabolites were mainly enriched in protein digestion and absorption,central carbon metabolism in cancer,ABC transporters,aminoacyl tRNA biosynthesis and biosynthesis of amino acids pathway;(6)The gene expression of phgdh in the liver of IUGR newborn piglets was significantly lower than that of normal newborn piglets(P<0.05);The protein expression of ATF4 was significantly lower than that of IUGR newborn piglets(P< 0.05).Experiment 2: 12 Twelve litters of ternary hybrid newborn piglets[Duroc×(Landrance×Yorkshire)]meeting the selection criteria of normal birth weight and IUGR were selected.According to the selection method of one normal male piglet and one IUGR male piglet in each litter,12 normal piglets and 12 IUGR piglets were selected.Under the same feeding environment and free lactation conditions,IUGR piglets were given 0.8%ser solution on the 1st,4th,7th,11 th,15th,19 th,22nd and 26 th days,and normal piglets were given the same volume of normal saline for 28 days,At the end of the test,slaughter sampling analysis was carried out after weighing and blood collection.The results showed that:(1)there was no significant difference in 28 day body weight between ser group and normal piglets(P>0.05);(2)There was no significant difference in liver index between ser group and normal piglets(P>0.05),and the pathological changes of liver were alleviated;(3)There was no significant difference in serum ALT and AST between ser group and normal piglets(P>0.05);(4)Serum free met and ile in ser group were significantly lower than those in normal piglets(P<0.05),while Gly,Val,thr and Leu had no significant difference with normal piglets(P>0.05);(5)A total of 43 differential metabolites(22 positive ion differential metabolites and 21 negative ion differential metabolites)were screened from serum samples of piglets and normal piglets in ser group.The differential metabolites were mainly concentrated in central carbon metabolism in cancer,protein digestion and absorption and histidine metabolism pathways;(6)The gene expression of phgdh,PSAT,PSPH and shmt1 in the liver of piglets in ser group were significantly lower than that of normal piglets(P<0.05),and the protein expression of shmt2 was significantly higher than that of normal piglets(P<0.05);(7)MDA in liver tissue of piglets in ser group was significantly higher than that of normal piglets(P<0.01),and the protein expression of ATF-4 was significantly lower than that of normal piglets(P <0.05);(8)The protein expression of TFAM and car in liver tissue of ser group was significantly higher than that of normal piglets(P<0.05),the protein expression of p-ampk was significantly higher than that of normal piglets(P<0.01),and ATP was significantly lower than that of normal piglets(P<0.05).In conclusion,IUGR newborn piglets have lower birth weight,impaired liver development and dysfunction of liver amino acid metabolism than normal newborn piglets,but ser can effectively alleviate the impact of IUGR on piglets and make IUGR piglets reach the level of normal piglets through catch-up growth,but ser can not completely alleviate the oxidative stress and metabolic function damage of IUGR piglets’ liver. |
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