| The purpose of this study was to explore the differences in the bacterial killing effect and the degradation of porcine circovirus II nucleic acid by commonly used disinfectants in pig farms in different scenarios.In this study,imported potassium hydrogen persulfate compound powder(A),domestic potassium hydrogen persulfate compound powder(B),caustic soda(C),and glutaraldehyde(D)commonly used in pig farms were selected on the surface of the ground feces(Scenario 1),ground manure after dry cleaning(Scenario 2),foot basin at the entrance of the pig house(Scenario 3),storage tank for the sewer of the pig farm(Scenario 4),disinfection pool at the entrance of the pig farm(Scenario 5),outdoor vehicle tires The sterilization effect was evaluated in the feces(scenario 6),the feces on the outdoor open ground(scenario 7),the feces on the indoor ground(scenario 8),and the urine on the indoor ground(scenario 9).Scenarios 3,4,5,6,and 7(outdoor group)enter the field according to the current outdoor temperature.Scenarios 1,2,8,and 9(indoor group)set three temperatures,which are 4℃,25℃,and 37℃,respectively.In scenarios 1,2,and 3,disinfectants A,B,and C were used for disinfection,and samples were taken at different time points,and the number of bacteria in the samples was determined by CFU counting.In scenarios 4 and 5,disinfectants A,B,and C are used for disinfection,and the liquid medium is cultured to observe whether the liquid medium becomes turbid.Mix the porcine ring type II virus liquid and feces and apply it to scenes 6,7,and 8,respectively.Mix the pig ring type II virus liquid with urine and put it in scene 9.Use disinfectants A,B,C and D disinfect the above four scenarios,take samples at different time points,and use real-time fluorescence quantitative PCR to detect the relative content of viral nucleic acid.The results show that,in scenario 1,after 1,5,15,and 30 minutes of disinfection by disinfectants A,B,and C,the bacterial killing rate on the surface of the fecal ball:Disinfectant A(4℃,33.38%-85.30%;25℃,62.90%-97.37%;37℃,38.75%-90.00%),Disinfectant B(4℃,67.30%-81.79%;25℃,55.63%-66.89%;37℃,54.70%-61.90%);Disinfectant C(4℃,99.59%-99.77%;25℃,65.32%-99.90%;37℃,99.31%-99.90%);in scene 2,after 15 and 30 minutes of disinfection with disinfectants A,B,and C,the bacterial killing rate in dung slag: Disinfectant A(4℃,95.49%-97.14%;25℃,99.27%-99.83%;37℃,99.94%-99.95%),Disinfectant B(4℃,99.05%-99.86 %;25℃,99.91%-99.95%;37℃,97.78%-99.91%);Disinfectant C(4℃,99.94%-99.95%;25℃,99.96%-99.97%;37℃,99.97%-99.98 %);in scene 3,after 5,15,and 30 s of disinfectant A,B,and C,the bacteria killing rate on the sole: Disinfectant A(84.16%-98.78%),Disinfectant B(85.48%-98.04%));Disinfectant C(82.52%-97.24%);In scenario 4,the time when the liquid medium began to grow bacteria: Disinfectant A(5 days),Disinfectant B(8 days),Disinfectant C(3 days);In scenario 5,the time for the liquid medium to grow bacteria: Disinfectant A(9 days),Disinfectant B(13 days),Disinfectant C(5 days);Nucleic acid negative time: Scenario 6,Disinfectant A(7h),Disinfectant B(7h),Disinfectant D(5h);Scenario 7,Disinfectant A(>7h),Disinfectant B(>7h),Disinfectant D(>7h);Scene 8,Disinfectant A(4℃,>7h;25℃,5h;37℃,>7h),Disinfectant B(4℃,>7h;25℃,7h;37℃,>7h);Disinfectant D(4℃,>7h;25℃,7h;37℃,5h);Scene 9,Disinfectant A(4℃,>7h;25℃,>7h;37℃,3h),Disinfectant B(4℃,>7h;25℃,3h;37℃,3h);Disinfectant C(4℃,>7h;25℃,>7h;37℃,>7h);Disinfectant D(4℃,>7h;25℃,>7h;37℃,3h).Conclusion: Disinfectant with better effect(recommended): use caustic soda for disinfection on the surface of the feces after the group transfer(scenario 1)and the residual manure on the ground after drying the feces(scenario 2);step on the basin at the entrance of the pig house(scenario 3)Use imported potassium persulfate compound powder for disinfection in pig farms;domestic potassium persulfate compound powder is used for disinfection in pig farm sewer storage tanks(scenario 4)and pig farm gate disinfection pool(scenario 5);outdoor vehicle tire manure(scene 6)use glutaraldehyde for disinfection;in outdoor open ground excrement(scenario 7),use imported potassium persulfate compound powder for disinfection;in indoor ground excrement(scenario 8),use imported potassium persulfate compound in spring and autumn In summer,glutaraldehyde is used for disinfection;in indoor floor urine(scenario 9),domestic potassium persulfate compound powder is used for disinfection.Significance:This study provides an effective clinical basis for farm production practice,and has practical significance for establishing and improving disinfection procedures,guiding scientific production,and using disinfectants effectively and rationally. |
