Study On Embedding Process And Stability Of Lactobacillus Plantarum And Enterococcus

This experiment aims to solve the problem of poor stability of lactic acid bacteria Preparations,from the selection of protective agent consumables to the technical control of the entire processing flow,to study the processing of lactic acid bacteria embedding technology(protective agent and carrier),and to develop a set of high stability lactic acid bacteria processing.The technology improves the survival rate and stability of lactic acid bacteria,and provides a new research idea for the development and utilization of lactic acid bacteria for feeding.Through the analysis of strain growth curve,acid production capacity and bacteriostatic effect,one strain of Lactobacillus plantarum and Enterococcus faecium with superior performance was screened for small-scale fermentation test.The small-scale fermentation test was divided into one control group(bacterial powder group)and nine test groups(A,B,C,D,E,F,G,H,I,respectively),9 According to the type and nature of the protective agent,different test agent formulations were designed according to the type and properties of the protective agent.The embedded products were made according to the relevant formulas.The storage stability of each group was investigated at different temperatures,and the best one was selected by statistical analysis.The protective agent formula was tested in the pilot test;the pilot test was carried out in the 1T tank fermentation production by the fermentation workshop of the Changping pilot test base of the National Institute of Food and Material Reserve,and the two best protective agent formulations obtained from the above-mentioned small test were pilot tested.Production,four products were Enterococcus faecalis powder,entrapped Enterococcus faecium,Lactobacillus plantarum powder and embedded Lactobacillus plantarum,respectively,the four products were tested for stress resistance,and the following results were obtained:(1)The embedding process of Lactobacillus plantarum RS048 was optimized.According to its storage stability at different temperatures,an optimal protective agent formulation was selected.The ratio of raw materials was 10% trehalose and 5% protein β.2% antioxidant beta and 1% inorganic salt beta.The stress resistance evaluation test showed that the embedded Lactobacillus plantarum had certain tolerance to high temperature,and the survival rate of the embedded product at 65 ℃,75 ℃ and 85 ℃ could still reach more than 55%;The gastric juice was poorly tolerated.After treatment for 4 hours in gastric juice,the survival rate of the powder was only 10.06%,and the survival rate of Lactobacillus plantarum was 121.87% higher than that of the powder.The difference was extremely significant(P < 0.01).Lactobacillus plantarum RS048 is more tolerant to intestinal fluid.After treatment for 4 hours in simulated intestinal fluid,the survival rate of the powder is kept above 60%,and the effect of embedding Lactobacillus plantum is obvious compared with the bacterial powder,and the difference is extremely significant(P < 0.01).(2)The embedding process of Enterococcus faecium RS047 was optimized.According to its storage stability at different temperatures,an optimal protective agent formulation was selected.The ratio of raw materials was 10% trehalose and 5% skim milk powder.2%antioxidant alpha and 1% inorganic salt alpha.The stress resistance evaluation test showed that the entrapped Enterococcus faecium had certain tolerance to high temperature,and the survival rate of embedded products at 65 ℃,75 ℃ and 85 ℃ could still reach more than 60%;Enterococcus faecium The gastric juice was well tolerated.After 4 hours of treatment in gastric juice,the survival rate of the powder was still above 55%,and the survival rate of the entrapped Enterococcus faecium was 31.89% higher than that of the powder.The difference was extremely significant(P < 0.01).)Enterococcus faecium RS047 is more tolerant to intestinal fluid.After 4 hours of treatment in simulated intestinal fluid,the survival rate of bacterial powder is still above 55%,and the effect of entrapped Enterococcus faecium is more obvious than that of bacterial powder.The difference is extremely significant(P < 0.01).In summary,this experiment obtained two kinds of protective agent formulations which can significantly improve the stability and stress resistance of Lactobacillus plantarum RS048 and Enterococcus faecium RS047.The best protective agent formula of Lactobacillus plantarum RS048 is 10% trehalose,5% protein β,2% antioxidant β and 1% inorganic salt β,and the optimal protective agent formula of Enterococcus faecium RS047 is 10% trehalose.5% protein gamma,2% antioxidant alpha and 1% inorganic salt alpha.