Research On The Function Of PEBP Family Genes RCN And OsMFT In Response To Stress In Rice

The PEBP gene family is highly conserved in evolution and is one of the most important gene families during plant development.In rice,the FT-like subfamily of the PEBP gene family is involved in the regulation of floral transformation and panicle architectural development.However,the role of TFL1-like and MFT-like subfamily in rice development is still unclear.In this study,the function of RCN of TFL1-like and OsMFT1 and OsMFT2 genes of MFT-like were explored.In this study,by creating RCN and OsMFT1,OsMFT2 related genetic materials,combining different environmental factors to explore their impact on yield and response to the environment.The results of this study reveal the diverse functions of members of the PEBP family in rice response to the external environment for heading,yield formation,seed dormancy and germination.The main findings include:1.RCN affect rice yield.The number of spikelets per panicle of rcn1,rcn3,rcn1 rcn3,rcn1 rcn2 rcn3 rcn4 decreased,the thousand-grain weight increased,and the yield decreased.The number of spikelets per panicle of rcn2 single-mutant material increased,the number of effective spikes decreased,the seed-setting rate decreased,and the yield decreased.The number of spikelets per panicle of rcn4 single-mutant material increased,the seed-setting rate increased,and the yield remained unchanged.The total spikelet number and thousandgrain weight of rcn2 rcn4 double-mutant materials increased,the number of effective panicles decreased,and the yield remained unchanged.2.RCN affect rice panicle traits.The number of branches and spikelets of the rcn1 rcn3,rcn1 rcn2 rcn3 rcn4 mutants decreased,resulting a smaller panicle.The number of branches and spikelets in the rcn2 mutant increased;the number of secondary branches of the rcn4 mutant increased,but the number of secondary spikelets decreased.3.RCN affect rice plant height.The plant height of rcn mutants was reduced,and the four-mutant material of rcn1 rcn2 rcn3 rcn4 became shorter.The four genes of RCN1-RCN4 may have additive effects.4.RCN are involved in the regulation of rice heading date by a variety of environmental factors.After the RCN mutation,the heading time of rice is earlier,and RCN1 and RCN3 have the greatest impact on the heading time.The rcn1 rcn3 double-mutant materials are more sensitive to short-day treatment than the wild type,and the short-day treatment with shorter days can induce heading,and the heading duration is significantly shorter than that of the wild type.rcn1 rcn3 are also involved in the regulation of drought and nitrogen on rice heading.Under drought and high nitrogen conditions,the delayed heading effect of rcn1 rcn3 is slower than that of the wild type.5.The OsMFT gene is involved in regulating the dormancy of rice seeds.The osmft2 mutant showed a large area of pre-harvest sprouting under the natural conditions of the field,and the osmft1 and osmft2 double-mutant materials were more obvious.6.OsMFT gene is involved in regulating the germination of rice seeds tolerant to salt stress.Under the salt stress,the germination rate of osmft1 single mutant was significantly higher than that of the wild type,and with the increase of salt concentration,the difference in germination rate between materials was more significant,indicating that OsMFT1 is a negative regulator of rice seed germination under the salt stress.The results of Quantitative Real-time PCR showed that:(1)The Salt stress can induce the expression of OsMFT1 and OsMFT2;(2)OsMFT2 may act on the upstream of OsMFT1;(3)OsMFT1 promote the expression of OsMFT2,OsMFT2 inhibits the expression OsMFT1.In summary,this study revealed that RCN-RCN4 play an important role in plant height,heading date and yield formation,and RCN1 and RCN3 respond to various external environmental factors.In addition,OsMFT1 is a negative regulator of rice seed germination under salt stress conditions.