| Wheat is a major grain crop in our country,so ensuring wheat production has great significance to protect national food security.Fusarium head blight(FHB)is a fungal disease caused by Fusarium graminearum,which is a serious blight on wheat head.In recent years,it has been endemic in a large area in Huang-Huai River winter wheat region,seriously affecting the yield and quality of wheat.In order to cultivate wheat varieties resistant to FHB suitable for planting in the Huang-Huai River winter wheat region.It is one of the important tasks of wheat breeding in this area.In this study,Sumai 3 were used as resistance material for FHB and a batch of intermediate materials carrying resistance gene of resistance were produced by crossbreeding combination with 979-5.Excellent breeding materials(k10,k12,k13,k31,k33)were selected as parents.375 high generation wheat lines were bred with the new varieties Xinong 20,Xinong 235,Xinong 282 and so on.Molecular marker-assisted selection was performed by closely linked with Fhb1,Fhb2,Fhb5 and QFhs.crc-2DL genes.Twenty-two new lines with excellent agronomic characters and resistance were selected based on single flower inoculation of field gibbodium spore suspension,identification of comprehensive agronomic characters and seed analysis of grain characters,which provided reference for wheat breeding of resistance to FHB.1.There are 8 pairs of primers were selected in this experiment,named Ta HRC-GSM for detecting Fhb1 gene,Wmc398 and Barc101 for detecting Fhb2 gene,Gwm154,Mag9482 and Gwm304 for detecting Fhb5 gene.The molecular markers Gwm539 and Wmc41 at QFhs.crc-2DL were detected.These markers were different between the parents and the positive control material(Sumai 3),and could be used to detect resistance genes.2.The results of FHB resistance gene /QTLs molecular markers were found in 375 high generation strains tested,there are 17 of all high generation lines carried Fhb1 gene,and 16 of them had medium resistance level or above,which further proved that Ta HRC-GSM was an effective selection marker for Fhb1.The resistance level of these 17 lines to FHB was improved compared with their male counterparts,indicating that the introduction of Fhb1 gene could effectively improve wheat resistance.Another line showed moderate resistance,indicating that Fhb1 gene should not be the sole basis for wheat resistance to FHB,but should be combined with field inoculation identification method.3.Among the FHB resistant lines,22 of the 27 resistance strains carried two or more resistance genes.Among 69 medium-resistant strains,54 strains carried two or more resistance genes.79.2% of the strains with medium resistance level or above were polymerized with two or more resistance genes,indicating that the polymerization of multiple resistance genes could improve the resistance of wheat.4.Among the FHB resistant strains,7 lines(67,155,248,316,331,333 and 368)were simultaneously resistant to stripe rust,and among the moderately resistant strains,14 lines(24,60,79,80,90,92,93,121,127,158,272,282,334 and 336)were simultaneously resistant to stripe rust.Six lines,67,93,316,331,333 and 368,carried Fhb1 gene.The main agronomic traits of these 22 lines could basically meet the main breeding objectives in the southern wheat area of the Huang-Huai River,and they were also resistant to FHB and stripe rust,which provided the basis for further selection and utilization of new wheat lines. |
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