Construction And Application Of Integrated Control Technology System For Ectropis Grisescens

Ectropis grisescens（warren）is one of the main pests in Chinese tea gardens,which seriously affect the production of tea.It has significant meaning to construction an integrated control technology system for E.grisescens to protect yield and quality of tea.This paper will study the integrated control technology system from three aspects:prediction,sex pheromone application and pesticide control.1.The life table of E.grisescens was established under the conditions of 22,26,30,34 and 38℃, photoperiod of 14L∶10D,and RH:70±10%.The developmental threshold temperatures of E.grisescens from egg to adult were 13.63,5.12C,3.82 and15.02℃,the effective accumulated temperatures were 58.92,322.92,199.62 and 79.36 degree-day,respectively.The regression prediction equations were:Y=0.315x²-17.804x+281.36（R²=1.00）,Y=-1.4227x+71.763（R²=0.79）,Y=-37.46ln（x）+156.52（R²=0.83）,Y=95.704e-0.039x（R²=0.80）.2.The adult population dynamics of E.grisescens was monitored by sex pheromone trap in the field,and the field monitoring data were used to verify the prediction data of regression prediction method and effective accumulated temperature method.The results showed that the polynomial regression prediction in the regression prediction method was more accurate than that of other prediction methods.The polynomial regression equation of the forecasting model was Y=0.315x²-17.804x+281.36.3.It was found that the male antennae of E.grisescens has electrophysiological respond to Z3,epo6,Z9-18:H and Z3,epo6,Z9-19:H’s isomer by GC-EAD.Subsequently,to evaluate synergy of four isomers of Z3,epo6,Z9-18:H and Z3,epo6,Z9-18:H,trapping effects were investigated in field.The males of traps were66.67±4.91,102.67±9.67,153.67±7.22,50.00±5.13,35.67±9.53 and 111.00±24.83 by sex pheromone,sex pheromone mixed with epo3,Z6,Z9-18:H,sex pheromone mixed with Z3,Z6,epo9-18:H,sex pheromone mixed with epo3,Z6,Z9-19:H and sex pheromone mixed with Z3,Z6,epo9-19:H,respectively.The results showed that the amount of Z3,Z6,epo9-18:H,Z3,Z6,epo9-19:H mixed with the sex pheromone was significantly higher than that of lure and the combination of epo3,Z6,Z9-18:H or epo3,Z6,Z9-19:H and lure.The results of mass trapping showed that the population of E.grisescens in the treated plot was significantly lower than that of the control plot.The mass trapping by sex pheromone could reduce the population of E.grisescens in the tea garden.4.The spinetoram,chlorfenapyr and spinosad were screened against the larva of E.grisescens in the light of toxicity,field control efficacy,safety evaluation to Arma chinensis and pesticide residues.The spinetoram demonstrated good contact toxicity and stomach toxicity activity against E.grisescens,with LC₅₀values of 9.16 mg·L^-1 and 0.31 mg·L^-1 respectively,which field control efficacy reached to 90%after 3 days and the pesticide residues was 0.00 mg·kg^-1 after 7 days.The LC₅₀of contact toxicity activity of chlorfenapyr of A.chinensis were 5.39 mg·L^-1.The LC₅₀values of contact and stomach toxicity of chlorfenapyr were 16.93mg·L^-1 and 176.00 mg·L^-1 respectively,which field control efficacy reached to 82.23%and the pesticide residue was 0.00 mg·kg^-1 after 7 days.The contact toxicity of LC₅₀ against A.chinensis was 116.54 mg·L^-1.The LC₅₀ values of contact toxicity and stomach toxicity of spinosad were 9.68 mg·L^-1 and 0.007 mg·L^-1,which field control efficacy reached to 82.81%after 3 days,the pesticide residue was 0.00 mg·kg^-1 after 7days.The contact toxicity of LC₅₀ against A.chinensis was 39.71 mg·L^-1.In this paper,it is verified that the occurrence period of E.grisescens in the field can be accurately predicted by regression prediction method and effective accumulated temperature method.On the basis of indoor GC-EAD,it is verified that the isomer of 18epo6 in the sex pheromone of E.grisescens has obvious synergistic effect.It is proved that the cylindrical trap is currently unable to replace the boat trap for routine field operations,and the experimental results conjecture that the method of using a large number of sex pheromone trapping can effectively reduce the population density of E.grisescens in the field.In the peak period,the field population can be rapidly reduced by using insecticides,so as to achieve the purpose of pesticide reduction,which can effectively reduce the pesticide residues of tea species.