| Wild soybean is a typical plant with high resistance,which has no reproductive isolation from cultivated soybean and is an excellent gene source of cultivated soybean.In this study,the glyphosate resistance capacity of 48 wild soybean materials was evaluated by culture dish identification and soil culture identification at the bud and seedling stages of wild soybean materials.By measuring the germination potential,germination rate,hypocotyl length,plant height,and main root length of wild soybean materials during the seedling stage,as well as the plant height,hypocotyl length,main root length,fresh stem mass,compound leaves per plant,dry stem mass,fresh aboveground mass,and dry aboveground mass of wild soybean materials during the seedling stage,combined with correlation analysis,relative value analysis principal component analysis and membership function analysis were used to calculate the comprehensive scores of wild soybean test materials in soil culture identification method and culture dish identification method.System clustering was used to grade the glyphosate tolerance of wild soybean test materials,and a comprehensive evaluation system for glyphosate tolerance of wild soybeans.Subsequently,a comparison was made between the methods for identifying the glyphosate tolerance of wild soybean materials,and the most suitable evaluation period and method for identifying the glyphosate tolerance of wild soybean materials were determined.Finally,by comparing the genomes of 200 wild soybean materials with 10× deep resequencing to explore wild soybean genotype data.The main results are as follows:1.Soil culture identification method: After principal component analysis,the identified trait indicators were divided into four principal components,with a cumulative contribution rate of 85.684%.The comprehensive score range of glyphosate tolerance was 0.123～-0.029.2012-42 had the highest comprehensive score of glyphosate tolerance and the lowest was 2005-64.2.Petri dish identification method: After principal component analysis,the identified trait indicators were divided into two principal components,with a cumulative contribution rate of 90.707%.The comprehensive score of glyphosate tolerance ranged from 0.268 to 0.965,2010-47 had the highest comprehensive score of glyphosate tolerance and the lowest was 2011-90.When the concentration of glyphosate solution reached 28mmol/L,The germination potential and germination rate of wild soybean materials under glyphosate stress showed the greatest significant difference compared to the control(0mmol/L).3.By analyzing the comprehensive scores of glyphosate tolerance in two identification methods,it was selected that the 2010-47(1.086)had the highest comprehensive score of glyphosate tolerance,while the 2011-90(0.244)had the lowest comprehensive score of glyphosate tolerance.The ranking of highly resistant and sensitive materials obtained by the culture dish identification method is basically consistent with the comprehensive ranking.4.A total of 533890 SNP loci were identified in the genome of 200 wild soybean materials.Based on these SNPs,the phylogenetic relationships of 200 wild soybean materials were analyzed,and it was found that the 200 wild soybean materials had distant phylogenetic relationships and were suitable for mapping glyphosate resistance genes as a natural population.The SNPs closely linked to glyphosate resistance genes were studied by genome-wide association study.The results showed that 17 SNP markers were closely linked to the target traits on chromosomes 1,3,4,8,9,16,19,and 20.Through SNP markers closely linked to the target traits,some resistance candidate genes were preliminarily identified. |
