| Auricularia heimuer,as a kind of large-scale glial fungus with both medicinal and edible value,has a history of thousands of years.In recent years,with the improvement of People’s living standard,more and more people gradually attach importance to the health of diet,and black fungus,as a kind of good-breath saprophytic fungus homologous to medicine and food,can not only meet people’s needs for delicious food,but also satisfy people’s needs,at the same time can be enough to prevent hypertension and hyperlipidemia and other common diseases.As one of the most important stress factors of adversity stress,drought stress has a great influence on Auricularia heimuer physiological activity.Therefore,in order to solve the adverse effects of drought stress on the growth and development of Auricularia heimuer fruit body,the antioxidant effects of Auricularia heimuer fruit body under different air humidity were studied,malondialdehyde(MDA)and the antioxidant capacity of enzymatic and non-enzymatic antioxidant systems of Auricularia heimuer MN149 at different air humidity levels were determined and statistically analyzed by biostatistics,it provides a theoretical reference for further study on the mechanism of antioxidant capacity in response to air humidity,and lays a foundation for future research on drought resistance in Auricularia heimuer fruiting body.The fruit body of Auricularia auricula were collected at air relative humidity of95%,90%,80%,70%,60% and 50%,respectively,and malondialdehyde(MDA),six kinds of enzyme antioxidants and three kinds of non-enzyme antioxidants were determined:(1)The air relative humidity was lower than 90%,the MDA content was increased,the membrane peroxidation appeared in the cell membrane system of Auricularia auricula fruit body,auricularia auricula fruit body was subjected to humidity stress.(2)The relative humidity of the air is below 90%,and the activities of SOD,CAT and POD increase,which constitute the first line of defense against humidity stress in the Superoxide dismutase of Auricularia auricula Catalase.(3)The content of ascorbic acid(ASA)was only higher in air relative humidity(80%),which constituted the first defense line of Auricularia auricula against humidity stress.(4)the relative humidity of the air was below 80%,and the activities of Glutathione reductase,mitochondrial(GR),Glutathione peroxidase(GSH-PX)and the contents of Glutathione(GSH)were higher,which constituted the second line of defense of Auricularia auricula against humidity stress.(5)the content of melanin was only higher in air relative humidity(70%),which constituted the second line of defense of Auricularia auricula against humidity stress.(6)ascorbic acid Peroxidase(APX)can not help the Auricularia auricula to resist humidity stress. |