Establishment Of Organogenetic System Of Three Chenopodium Quinoa Varieties

Chenopodium quinoa Willd.is a halophyte with a comprehensive nutritional composition,which has attracted much attention in genetic breeding in recent years.The establishment of a complete organogenetic system can provide a receptor for genetic transformation and a rapid propagation system for quinoa gene research and transgenic breeding,while the establishment of a specific organogenetic system for the existing varieties in the laboratory can improve the efficiency and quality of the experiment.The organogenetic systems of three varieties of Quinoa,Faro,Dave and NL-6,are established in combination with previous laboratory studies.The establishment of indirect organogenetic system includ callus induction,proliferation and differentiation of Faro and selection of the best methods for embryogenic callus induction.Selection of best methods for callus induction and proliferation of varieties Dave and NL-6.Establishment of direct organogenetic system includes the best methods for axillary bud induction,proliferation and rooting of varieties Dave and NL-6.Specific research results are as follows:1.The optimal callus induction formula of Quinoa Faro explants was different.The cotyledon explants were MS+4.0mg/L 6-BA+1.0mg/L NAA,and the cotyl explants were MS+0.5mg/L 2,4-D+0.05mg/L 6-BA.Seed embryo explants were MS+0.2mg/L2,4-D+0.5mg/L ZT,and young panicle explants were MS+0.5mg/L 2,4-D+0.5mg/L6-BA.Cotyledon explants and formula MS+4.0mg/L 6-BA+1.0mg/L NAA were selected as the callus induction scheme for indirect organogenesis of Faro.The best formula for indirect organogenic callus proliferation of Faro was MS+4.0mg/L6-BA+0.5mg/L NAA.The best formula for callus differentiation was MS+1.0mg/L TDZ.Appropriately increasing sucrose concentration can be a feasible method to induce embryogenic callus development.2.The optimal formula for callus induction of cotyledon explants and cotyls of quinoa variety Dave is MS+0.5mg/L 2,4-D+0.2mg/L 6-BA.The best formula for callus proliferation in indirect organogenesis of variety Dave was MS+0.1mg/L2,4-D+0.05mg/L 6-BA.3.Different explants of Quinoa variety Dave had different formulas for callus induction.The cotyledon explants were MS+0.5mg/L 2,4-D+0.5mg/L 6-BA,and the cotyl explants were MS+0.5mg/L 2,4-D+0.2mg/L 6-BA.The best formula for callus proliferation in indirect organogenesis of variety Dave was MS+0.1mg/L2,4-D+0.2mg/L 6-BA.4.The axillary bud induction scheme of quinoa variety Dave was as follows:cotyledon with terminal bud and 1 cm cotyl was used as explants,and the optimal induction formula was 2MS+1.0mg/L 6-BA.The optimal formula for axillary bud proliferation was 2MS+1.0mg/L 6-BA.The optimal rooting formula was2MS+1.0mg/L IBA.5.The axillary bud induction scheme of Quinoa variety NL-6 by direct organogenesis was as follows: cotyledon with terminal bud and 1 cm cotyl was used as explants,and the optimal induction formula was 2MS+1.0mg/L 6-BA.The optimal formula for axillary bud proliferation was 2MS+2.0mg/L 6-BA.The optimal rooting formula was 2MS+1.0mg/L IBA.