Identification And Evolution Analysis Of HSF And HSP Gene Family In Brassica Napus

Brassica napus is a very important oil crop.Heat shock proteins(Hsps)and Heat shock transcription factors(Hsfs)are ubiquitous in various plants,and play an important role in the plant growth and defense against abiotic stress.To comprehensively and deeply understand the information of Hsf and Hsp gene families in B.napus,the subject mainly includes the following six aspects:(1)We identified and conducted bioinformatic analysis of Hsf and Hsp gene families of three Brassica species and Arabidopsis thaliana.A total of 95 Hsf,63 s Hsp,69 Hsp60,46 Hsp70,17 Hsp90 and 18 Hsp100 genes were identified in B.napus.According to phylogenetic tree topology and subcellular localization,the genes from each gene family were divided into different subgroups.The genes of B.napus are more closely related to Brassica rapa and Brassica oleracea than to A.thaliana on the evolutionary tree,which is consistent with the kinship of species.(2)The number of some Hsf and Hsp genes in B.napus is not equal to that of B.rapa and B.oleracea,suggesting that the gene loss or duplication occurred after hybridization and fusion of two diploids into tetraploid B.napus.(3)The analysis showed that gene structure and conserved motifs of whole family were quite different,but they had high consistency within the same subgroup.(4)Identification and statistical analysis of gene duplication types showed that whole-genome duplication or segmental duplication played a very important role in the expansion of Hsf and Hsp gene families.(5)Transcriptome analysis showed that most Hsf and Hsp family genes of B.napus were expressed in roots and leaves,among which Hsp70,Hsp90 and Hsp100 family genes were highly expressed.Some genes have obvious tissue-specific expression.(6)Differentially expressed Hsf and Hsp family genes were identified under high temperature,low temperature and drought stress in B.napus.Some genes respond to high temperature and other stresses at the same time.Co-expression network of Hsf and Hsp family genes was constructed,and we detected the core genes that may play a key regulatory role in the network.Figure 40;Table 27;Reference 121...