Cloning,expression Analysis Of SOD Gene In Phyllostachys Edulis And Functional Analysis Of PeFeSOD2 Under Salt Stress

Moso bamboo(Phyllostachys edulis)is one of the most important economic bamboo species in China,but it affected by abiotic stresses such as drought,salinity and low temperature,it is unevenly distributed and its yield is also greatly affected,and it also has a certain impact on the development and utilization of moso bamboo resources.Superoxide dismutase(SOD)is an important antioxidant enzyme for scavenging active oxygen in plants,and SOD gene family is a kind of gene encoding superoxide dismutase in plants,which can regulate the activity of superoxide dismutase in plants,and plays an important role in the process of resistance to stress.In this study,the SOD gene family of moso bamboo was selected as the research object,through bioinformatics analysis,cloning and expression analysis,and Pe Fe SOD2 was selected to further explore its function through transgenic methods.The main research results are as follows:1.According to the moso bamboo genome database,7 SOD genes were identified.An evolutionary tree was constructed among Oryza sativa and Arabidopsis thaliana SOD aimno acid sequences,and 3 subfamilies were obtained: Cu/Zn-SOD subfamily,Fe-SOD subfamily and Mn-SOD subfamily,the same subfamily of moso bamboo SOD genes have the same conserved domain.According to the analysis of cis elements of the promoter,the promoter of moso bamboo SOD gene is rich in elements related to resistance to stress and hormone response.2.According to the q RT-PCR,the expression of 7 moso bamboo SOD genes under different stress and hormone treatment were analyzed,it was found that 7 moso bamboos had different expression patterns under different stress and hormone treatment.According to the yeast two hybrid experiment,we found Pe CZSOD2,Pe Mn SOD2 and Pe Fe SOD1 proteins can interact with themselves,Pe Mn SOD1 and Pe Mn SOD2 can interact,Pe Fe SOD1 and Pe Fe SOD2 also can interact,it indicates that SOD protein of moso bamboo may play a role by binding to itself or the same subfamily SOD proteins to form multimer.It was also confirmed that Pe Fe SOD1 and Pe Fe SOD2 had interaction relationship with Pe BAS1,it indicates that Pe Fe SOD1 and Pe Fe SOD2 proteins may combine with Pe BAS1 protein to form multimers and play a role in resisting oxidative stress.3.In order to further study the function of SOD gene from moso bamboo,Pe Fe SOD2 gene was transformed into rice by heterologous transformation,and the overexpression rice of Pe Fe SOD2 were obtained.Under higher salt stress,the results showed that the growth of transgenic rice was better than the wild type rice,and the plant height,root length and superoxide dismutase activity of transgenic rice were also higher than the wild type rice,MDA of the transgenic rice was significantly lower than the wild type rice.Moreover,the expression of salt stress related genes in transgenic rice was up-regulated compared with the wild type rice according to q RT-PCR analysis.These results indicate that the SOD gene family of moso bamboo has a certain function in abiotic stress resistance.This paper provides a theoretical basis for elucidating the mechanism of bamboo resistance to abiotic stress.