Effects Of Different Antioxidants On Cryopreservation And Antioxidant Indexes Of Wapiti Sperm

Accelerating the research on the cryopreservation technology of wapiti semen is of great significance to the wapiti breeding industry.China began to study the technology of wapiti semen cryopreservation as early as 1960 s,and has made great progress,but the conception rate of frozen semen is still low,and the semen cryopreservation diluent still needs to be optimized.Recent studies have shown that adding antioxidants to semen cryopreservation diluent can improve the quality of frozen and thawed sperm.However,there are few reports on whether adding antioxidants to wapiti semen cryopreservation diluent can improve the quality of frozen and thawed sperm.Based on this,this study took Yihe wapiti as the experimental object to study the effects of different antioxidants(glutathione,trehalose,vitamin E and cysteine)on the cryopreservation effect and antioxidant indexes of wapiti semen,so as to provide data reference for the cryopreservation of wapiti semen.The main results of this study are as follows:1.Adding different concentrations of glutathione(5,7,10,15 mg/m L)to the diluent had a certain negative impact on sperm motility,deformity rate,lateral swing amplitude,linear rate,plasma membrane integrity rate,acrosome integrity rate and antioxidant enzyme activity in semen,and all sperm in the high concentration glutathione group(15 mg/m L)died.2.Adding different concentrations of trehalose(25,30,35,40 mg/m L)to the frozen diluent had a certain effect on the quality of frozen and thawed sperm.The sperm motility of 35 mg/m L trehalose group was significantly improved(P< 0.05),the plasma membrane integrity rate and SOD activity of sperm were significantly higher than those of the control group(P < 0.01),and the GSH-PX activity was slightly higher than that of the control group;The amplitude of sperm sway ALH in 25 mg/m L trehalose group increased,but there was no significant difference between 25 mg/m L trehalose group and the control group(P > 0.05);The sperm deformity rate decreased and the acrosome integrity rate increased in the 30 mg/m L trehalose group,but there was no significant difference between the 30 mg/m L trehalose group and the control group(P >0.05).3.The addition of different concentrations of vitamin E(0.2,0.4,0.6,0.8 mg/m L)to the frozen diluent had a certain effect on the quality of frozen and thawed sperm.Among them,the activity of SOD in 0.6 mg/m L vitamin E group was significantly increased(P < 0.01),the integrity rate of sperm plasma membrane was significantly increased(P < 0.05),and the sperm motility,acrosome integrity rate and CAT activity were increased,but there was no significant difference between 0.6 mg/m L vitamin E group and the control group(P > 0.05).4.Adding different concentrations of cysteine(1,2,3,4 mg/m L)to the frozen diluent had a certain effect on the quality of frozen and thawed sperm.The sperm motility,acrosome integrity rate and plasma membrane integrity rate in 3 mg/m L cysteine group were significantly increased(P < 0.01);The amplitude of sperm sway increased and the rate of sperm deformity decreased in 1mg/m L cysteine group,but there was no significant difference between 1 mg/m L cysteine group and the control group(P > 0.05);The activity of GSH-Px in 2 mg/m L cysteine group increased,but there was no significant difference with the control group(P > 0.05);After adding cysteine,the linear rate decreased.The above results show that the addition of 35 mg/m L trehalose,0.6 mg/m L vitamin E and 3mg/m L cysteine to the frozen preservation diluent of wapiti semen can significantly improve the activity of SOD enzyme,protect sperm from oxidative stress and improve the quality of frozen thawed wapiti semen.