GWAS Of Soybean Resistance To Bean Common Mosaic Virus And Detection Of Bean Yellow Mosaic Virus By RPA-LFD

Soybean(Glycine max(L.)Merr.)annual herb,originated from China and is cultivated in many countries and regions around the world at present,serving as an important grain crop and oil crop.For a long time,soybean mosaic virus,as one of the important plant diseases and insect pests affecting soybean yield and quality,has been widely concerned.In recent years,Bean common mosaic virus(BCMV)has been widely spread and seriously endangered in various soybean producing regions in the world.In this study,4 strains from different subgroups were selected from BCMV strains isolated in the early stage of laboratory,and then preliminarily identified on 15 differential hosts;231 resequenced soybean germplasm resources from whole country were identied for resistance to bean common virus disease and genome-wide association studies,resistance resources and associated SNP sites were screened out;Bean yellow mosaic virus(BYMV)has a very high incidence in the field of broad bean and pea.Recombinase polymerase amplification(RPA)as a rapid and efficient detection method is essential for the prevention and control of bean yellow mosaic virus,RPA-LFD rapid detection method of BYMV were studied.The main research results are as follows:(1)Different isolate of BCMV have different susceptibilities to different soybean variety due to that strength of pathogenicity,The 4 BCMV lines of 19-RD03,20-RD05,20DH14 and 20-DT2 were divided on 15 differential hosts of ’Essex’,’PI96983’,’Suweon 97’,’York’,’Raiden’,’Kwanggyo’,‘Ogden’,’Marshall’,’PI507389’,‘L29’,’V94-5152’,’Nannong 1138-2’,’Williams 82’,‘Kefeng No.1’and ’Sudou 13’respectively.Classification and identification of resistance among strains showed that the phenotype of strain 20-RD05 was obvious and the pathogenicity was strong.(2)A total of 231 soybean natural population germplasm resources were identified using BCMV strain 20-RD05,and 78 high resistance and disease resistance germplasm resources were obtained.Using the resequencing data,genome-wide association study(GWAS)was performed on the disease resistance phenotype of soybean natural population,and 655 single nucleotide polymorphism(SNP)loci related to the resistance of 20-RD05 virus strain on chromosomes 2,3,7,9,10,14,15 and 18 were associated,and 8 SNPs with the most significant correlation with disease resistance were screened out,which laid the foundation for the subsequent mining of disease resistance genes.(3)We designed a specific primer and probe based on recombinase polymerase chain reaction(RPA)detection technology and combined with lateral flow strip(LFD)to detect the CP gene of bean yellow mosaic virus(BYMV),and established a rapid RPA-LFD detection method for B YMV.In the experiment,the sensitivity of the method was compared with that of PCR technology,and the strong specificity was verified.At the same time,the field samples could be detected within 30 minutes,with accurate results and convenient operation.