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Characteristics Of Ectomycorrhizal Fungi Community Of Pinus Massoniana And Screening Fungi-Tree Combinations For Drought Tolerance And Phosphorus Absorption

Posted on:2023-11-24Degree:MasterType:Thesis
Country:ChinaCandidate:J X HaoFull Text:PDF
GTID:2543306851487224Subject:Silviculture
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Pinus massoniana is the main afforestation tree species in southern China.In recent years,with the aggravation of Rocky Desertification,multiple drought and lack of phosphorus in the south,the yield of Pinus massoniana forest is reduced and its growth is limited,which seriously restricted the development of timber forest and resin forest of Pinus massoniana.In order to cope with this phenomenon,Hunan Academy of Forestry introduced a batch of Root Production Method(RPM)seedlings,which have achieved good results after afforestation in rocky desertification mountainous areas in this area,but the number of introduced seedlings is limited and the price is expensive,which is a thorny problem.Therefore,this study attempts to compare the community structure characteristics of Ectomycorrhizal fungi(ECMF)around the roots of Pinus massoniana conventional seedlings and RPM seedlings by Illumina miseq sequencing technology,in order to understand the advantages of RPM seedlings over conventional seedlings.At the same time,4-month-old Pinus massoniana was artificially inoculated with ECMF under laboratory conditions.The strains with more root circumference distribution of RPM seedlings were selected: Suillus Bovinus,Suillus luteus,Rhizopogon Luteolus,Pisolithus Tinctor are inoculated by single inoculation and mixed inoculation of the same amount of the above strains.set the normal water,drought stress and three different phosphorus concentrations treatment,Pure vermiculite is selected as the substrate to reduce the influence of culture substrate nutrients,after drought stress and different phosphorus concentrations treatment,measure different fungi-tree`s mycorrhizal infection rate,growth,physiological and biochemical indexes and soil physical and chemical properties,the purpose is to use the mycorrhizal biological advantages of RPM seedlings to improve the growth status of oridinary seedlings,screen excellent combinations of bacteria and trees that resist drought and promote phosphorus absorption,and provide a theoretical basis for the Directional Cultivation of Pinus massoniana fungi-tree.The results show that:(1)Using Illumina miseq sequencing technology,a total of 170148 sequences were detected from the root and root circumference soil samples of two Pinus massoniana seedlings,which were divided into 20 OTUs,belonging to 2 phyla,3 classes,7 orders,8families and 11 genera.(2)Genera with a proportion of >1% in the ECMF community are listed below,and the root samples of RPM seedlings are Amphinema,Tomentella and Tylospora.Conventional root samples were Tomentella and Tylospora.The soil samples of RPM seedlings were Tylospora,Suillus,Tomentella and Amphinema.Ordinary seedlings soil samples were Tomentella,Russula,Tylospora,Cenococcum,Tomentellopsis and Multifurca.Compared with ordinary seedlings,the unique genus of RPM seedlings is Suillus.(3)Redundancy analysis(RDA)found that soil p H value was the most significant influence on ECMF richness,followed by organic matter,available phosphorus and available nitrogen.The effects of soil physical and chemical properties on different ECMF genera in soil were significantly different.Organic matter,p H,available P and alkali nitrogen were negatively correlated with Tomentella,Cenococcum,Russula and Tylospora.Tomentellopsis,Amphinema,Multifurca and Suillus were positively correlated.(4)The inoculation treatment could form symbiotic relationship with seedlings,while the control treatment didn`t.After normal water and drought stress treatment,the inoculation treatment could form symbiotic relationship with seedlings,but the control treatment did not form mycorrhiza.After normal water and drought stress treatment,the morphology of each inoculation treatment was different.Drought stress under the condition of ECMF by improving the Pinus massoniana biomass,chlorophyll content,total N content and total P content,soluble sugar content,soluble protein content,SOD activity and POD activity to enhance the capacity of Pinus massoniana drought resistance,and under drought stress of soil organic matter and available N content is reduced,the content of available P and available K increased.(5)Under drought stress,Pinus massoniana inoculated with Suillus luteus was the strongest,followed by Rhizopogon Luteolus,Suillus Bovinus,Pisolithus Tinctor and mixed strains.(6)Under the condition of phosphorus deficiency,ECMF enhanced the anti-phosphorus fertility ability of Pinus massoniana by increasing biomass,chlorophyll content,total N content,total P content,soluble sugar content,soluble protein content,SOD activity and POD activity,and the soil organic matter,available N,available P and available K of inoculation treatment were higher than those of control.(7)Under different phosphorus concentrations,Pinus massoniana inoculated with Suillus luteus brown had the strongest ability to adapt to phosphorus deficiency environment,followed by Rhizopogon Luteolus,Suillus Bovinus,Pisolithus Tinctor and mixed strains.
Keywords/Search Tags:Pinus massoniana, Root production method, Ectomycorrhizal fungi, Drought resistance mechanism, Phosphorus absorption
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