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Effects Of Volatiles From Cones Of Pinus Koraiensis On Host Selection Of Important Cone Pests

Posted on:2023-05-02Degree:MasterType:Thesis
Country:ChinaCandidate:J F WangFull Text:PDF
GTID:2543306842973159Subject:Forest science
Abstract/Summary:PDF Full Text Request
With the increase of plantation area of Pinus koraiensis in Northeast China every year,the proportion of cone pests also increases.With the increase of chemical dosage demand of poisonous pests,forestry control needs to try new control measures.Dioryctria abietella,Dioryctria sylvestrella and Eupithecia abietaria debrunneata Staudinger are important pests of cones of P.koraiensis,and many pests occur in the same cone.In this study,the effects of cone volatiles on host selection preference of cone pests were investigated by component analysis of volatiles,as well as by combining with electroantennogram and olfaction behavior response tests.Scanning electron microscope was used to observe and analyze the types of antennal sensilla of E.abietaria debrunneata,so as to lay a theoretical foundation for ecological control of P.koraiensis forest.The main research results are as follows:(1)Through sampling different types of cones of P.koraiensis,it was found that there were37 species in 7 categories.The most volatile had 5 categories and 22 species in the damaged cones of D.abietella.The least volatile had 5 categories and 16 species in the damaged cones of Gravitarmata margarotana.Only the healthy cones in July contained special volatiles,which were3-methyl-2-heptanone,4-hydroxy-2-butanone,2,2,4,4-tetramethylpentane and Benzaldehyde.There were also significant differences in volatile content among different types of cones.(2)Through the olfactory response test,it was found that the females of D.sylvestrella preferred the damaged cones of D.sylvestrella,males of D.sylvestrella preferred the damaged cones of D.abietella.Both female and male of D.abietella preferred the healthy cones.females of E.abietaria debrunneata preferred the damaged cones of D.abietella,males of E.abietaria debrunneata preferred the healthy cones.(3)The EAG relative values of 10-3mol/l propylene oxide and 1 mol/l methyl tert butyl ether were significantly different between male and female adults of D.abietella.10-3~10-1mol/l of propylene oxide significantly repelled females,10-3mol/l of ethylbenzene and 10-3mol/l of l-caryophyllene significantly attracted females,10-3mol/l propylene oxide significantly attracted males.(4)The EAG relative values of 10-5mol/l myrcene,10-1mol/l(+)-Dipentene,1 mol/l3-carene and 1 mol/l l-caryophyllene were significantly different between male and female adults of E.abietaria debrunneata.10-1mol/l of myrcene significantly repelled females,10-2mol/l of3-carene significantly repelled males.(5)The antennal sensilla of E.abietaria debrunneata were observed by the scanning electron microscope.There were 17 species in 10 categories,including b(?)hm bristles(Ⅰ-Ⅱ),sensilla furcatea,sensilla squamiformia,sensilla trichodea(Ⅰ-Ⅲ),sensilla chaetica,sensilla basiconicum(Ⅰ-Ⅱ),sensilla auricillica(Ⅰ-Ⅲ),sensilla cavity,sensilla coeloconica(Ⅰ-Ⅱ)and sensilla styloconica.Among them,there were significant differences in the number of sensillum trichodeum(Ⅰ,Ⅲ)and sensillum chaeticum.
Keywords/Search Tags:cone volatiles, electroantennogram, olfactory behavioral response, antennal sensilla
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