Transformation And Drought Resistance Analysis Of PubZIP1 From Populus Trichocarpa In Larix Kaempferi

Larch plays the most important role in the economic and ecological fields as an important afforestation and economic species.Now,conventional breeding means are significantly less produced than economic needs due to their high cost and long cycle characteristics.It is important and urgent to destroy the balance of the ecosystem by global warming,and to increase the resistance of larch in an increasingly difficult survival environment.Therefore,the status of genetic engineering in forestry is becoming more important.Based on an efficient somatic embryo induction system,this study aims to genetically transform L.kaempferi using Agrobacterium-mediated method and performed the stress resistance analysis.The main research results are as follows:(1)Subcellular localization of the gene showed that PubZIP1 was located in the nucleus.The results of fluorescence quantitative PCR showed that the expression of PubZIP1 gene was the highest in root,the lowest in stem and the middle in leaf.Under drought stress,the expression of PubZIP1 gene increased in roots,and decreased after rehydration.(2)Acquisition of transgenic Larix kaempferi and plant regeneration.Using L.kaempferi embryogenic callus as the recipient material,the Agrobacterium-mediated method was used to carry out genetic transformation of PubZIP1 gene in Larix kaempferi,and obtained 3 resistant transgenic lines,and DNA levels and RT-PCR were tested.(3)Drought resistance analysis of transgenic Larix.Three transgenic L.kaempferi lines and WT were subjected to 7% PEG6000 stress,respectively.It was found that the transgenic plants suffered less damage than the WT.By relative water content,soluble sugar content,proline content and electrolyte leakage,these indicated the transgenic lines suffered less damage than WT.It demonstrates that the overexpression of PubZIP1 gene improved the drought resistance in L.kaempferi.