| The abuse of antibiotics and the illegal use of human medication occur frequently in pet clinics,resulting in increased drug resistance of Salmonella in dogs,and even the emergence of a large number of pan-resistant strains,which makes the current clinical treatment of Salmonella infections face severe challenges.Plasmids,as a mobile element,have the advantage of being able to replicate autonomously and to participate in the horizontal transfer of drug resistance in Salmonella.The integron is a common genetic element in Gram-negative bacteria,which can capture drug resistance genes and promote the generation and spread of bacterial resistance in terms of drug resistance.In this study,samples were collected by anal swab method to isolate and identify Salmonella pet dogs in Changchun area,and detect the drug resistance and drug resistance genes of pet canine Salmonella.and explore the relationship between integron and drug resistance genes.Research results:In this experiment,370 canine fecal samples were collected from 12 pet hospitals in Changchun,and 38 Salmonella strains were isolated and identified,and 20 antibiotics such as quinolones and aminoglycosides were selected to study the drug resistance spectrum and multidrug resistance of the isolated strains.The test results showed that the resistance rates of vancomycin and amoxicillin-clavulanate potassium were the highest,92.1%and 81.6%,respectively;followed by ciprofloxacin and ampicillin resistance rates were 76.3%and 73.7%,respectively;The drug results showed that the multi-drug resistance spectrum was wide,and the number of strains with multi-drug resistance or above accounted for 97.37%（37/38）.Experimental design 23 drug resistance genes such as sulfonamides andβ-lactams were used to study the correlation between drug resistance spectrum and drug resistance genes.The research data were quinolone resistance genes:aac（6′）-Ib-cr and qnr S The detection rates of,β-lactam drug resistance gene bla TEMand aminoglycoside resistance gene aph（3）-IIa were84.2%,81.6%,81.6%and 73.7%,respectively.Drug resistance was consistent with the results for drug resistance genes.Based on 38 Salmonella strains,class I,II,and III integrons were studied in the experiment,and the relationship between the integron-positive strains and the drug resistance spectrum was clarified through empirical research as the resistance spectrum of class I integron-positive strains.The detection rate was higher than that of class I integron-negative strains.The conjugation test was conducted on 38 Salmonella strains,and the correlation between drug resistance genes and integron was studied based on the conjugative strains.The gene was amplified,and the results showed that the detection rates of sul I,sul II,sul III,aac（6′）-Ib-cr,bla TEM,bla CTX-M,aad A2,aph（3）-IIa,tet M and tet B were all 100%;the detection rate of class I integrons in the conjugation strains was 100%,and class II and class III integrons were not detected.The results showed that some of the strains isolated in this study could be transmitted across species through plasmids carrying the above multiple drug resistance genes,and the class I integron played an important role in the drug resistance transmission of pet dog-derived Salmonella in Changchun area.At this stage,the research on the related issues of the drug resistance mechanism of Salmonella can provide reference for the prevention and treatment of Salmonella in pet dogs in Changchun area,the standardized application of antibiotics and the clinical treatment of pets. |
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