Influence Of L-Carnitine On The Oxidation Function Of Fat Tissue Based On NRF2 Signal Path

With the disabling of feed antibiotics,the role of green additives in livestock is more emphasis.L-Carnitine(L-carnitine,referred to as LC)is a green nutrition additive,which is an important carrier tool in the process of animal body fat and means for supplemental energy,which can alleviate the body’s oxidative stress,but its regulatory mechanism is not yet clear.This test aims to explore the effects of L-carnitine on the antioxidant function of hyperoxide fatty tissue by NRF2 signaling pathway,to clarify the oxidation of nutrients L-carnitine-free tissue,and lay the foundation for preventing fattening sheep oxidation Base.This test choice to use 24 body type,March,March,March-age,March,March,March,March,March(Xia Han F1),moon,as a test animal,randomly divided into 3 groups,each treatment group 8 fattening lambs,each repeating,ie control group(Control group,feeding TMR),low concentration L-carnitine group(L-LC group,feeding TMR + 0.01% lc),high concentration L-carnitine group(H-LC group,feeding TMR + 0.05% LC),feeding with roughness ratio 3: 7.Test pre-feeding period 15 d,formal period 45 d.By detecting the effect of L-carnitine on antioxidant indicators in the blood of Xia Han F1,the expression of antioxidant genes in adipose tissue,while comparing the difference in synthesis of adipose tissue.On this basis,the change of the anti-carnitine F1 generation of Xia Han is cultured in vitro,and the anti-oxidation effect of L-carnitine on the NRF2 signaling pathway of fattening lamb renalone fat cells is further verified.The effect of adding L-carnitine on antioxidant function of fattening lambs.The results showed that:1.Effects of adding L-carnitine on antioxidant indicators in hyperbatinous lambs.The results showed that the MDA content was significantly lower than the control group(P<0.01),while SOD activity was significantly higher than that of the control group(P <0.05),and GSH-PX activity was extremely significantly higher.The control group(P <0.01).These results indicate that L-carnitine can increase the antioxidant function of blood of Xia Han F1 generation.2.Differential differences in hypoglycine on fattening lamb fat tissue protein group showed that 57 upper-regulated protein expression were co-identified,and 303 low-profile proteins were expressed.Among them,the antioxidative stress-related passage includes fluid shear stress and protein processing,Parkinson disease,cancer pathway,chemical carcinogenic effects,lipid and arteries Porridge hardening.The protein expression of NRF2 signaling pathway is detected by Western Blotting.The protein expression of NRF2,HO-1,and KEAP1 was found that the addition of the left-lottonyzine group was significantly higher than that of the control group(P <0.01).The results show that the L-carnitine can improve the body antioxidant function by acting in the Hantan F1 endocytic tissue.3.The original precidential fat cell model was cultured in vitro,and L-carnitine treatment of fat cells was added,and the expression of NRF2 and KEAP1 protein expression was higher than that of the control group(P <0.01).The results show that the L-carnitine can improve the antioxidant capacity of adipose tissue by NRF2 signaling pathway.In summary,this study suggests that the L-carnitine can effectively improve the antioxidant capacity of hybrid sheep fat cells,reducing the effects of oxidative stress on the body,promoting the growth and development of summer cold F1.