The Function Of Prenyltransferase PsERG20 And PsRAM1 In Development And Virulence Of Phytophthora Sojae

Soybean root rot caused by Phytophthora sojae is widely distributed in Northeast province and Huang-huai-hai region of China and lead to enormous ecnomic loss.Loss of soybean resistance and rise of fungicide resistant strains make control of PRR more difficult.It is urgently needed to explore new chemical control targets and design new anti-Phytophthora chemical agents.Isopentenyl transferase catalyze the transfer of isopentenyl diphosphate(IPP),dimethylallyl diphosphate(DMAPP)for the synthesis of isoprenoids or protein post translational modification.In this research,the function of PsERG20 and PsRAM1 was investigated and the activity of two inhibitors was assessed.The main results were listed as follows:1.Lovastatin depress development,virulence of P.sojae and affect gene expression.Lovastatin target HMG-Co A reductase in MVA pathway,and lovastatin treatment inhibit growth of P.sojae,depress the production of sporangia and oospore,increase encyst rate of zoospores.The expression levels of genes in MVA pathway were also affected by lovastatin.2.PsERG20 regulate development and virulence of P.sojae.PsERG20 is one of the key gene in MVA pathway.Phylogenetic analysis indicated that PsERG20 had high similarity with animal homologous genes.The amino acid sequence similarity was 70.43%.Moreover,PsERG20 has high identity and similar protein structure with the homologues of Saccharomyces cerevisiae.PsERG20 located in cytoplasm and regulate growth,sporangia and oospore production and virulence of P.sojae.3.Bisphosphonates inhibit development and virulence of P.sojae.Nitrogen containing bisphosphonates(N-BPs)target PsERG20 and has high activity to P.sojae.N-BPs treatment depress growth,sporangia and oospore production and virulence of P.sojae.The results indicated N-BPs is another potential chemical drug lead for anti-Phytophthora fungicide.4.PsRAM1 regulate development and virulence of P.sojaePsRAM1 is a isopentenyl transferase which mediate the protein prenylation with FPP as substrat.Phylogenetic analysis indicated that PsRAM1 had high similarity with homologues from plants and yeast.The amino acid sequence similarity was 44.78%.Knock out of PsRAM1 lead to slow growth,reduced production of sporangia and oospores and virulence.The results indicated the importance of this gene and could be used as another control target.