Directional Breeding And Utilization Of Male Sterile Lines In Stay-green Pakchoi And Chinese Flowering Cabbage

To take advantage of Chinese flowering cabbage and stay-green pakchoi heterosis,initiative is 100%sterility strain rate of male sterile lines,solve the problem of hybrid seed production means,this test is to vegetables nuclear genetic male sterile line source for infertility,directional commercial design scheme,to the use of microspore culture technology create excellent vegetables DH system into nuclear sterile genes,creating excellent vegetables male nuclear gene Education department;The cytoplasmic male sterility line’618’was used as the sterility source,and the excellent cytological male sterility line’618’was used as the recurrent parent,directed transbreeding of excellent male cytoplasmic sterile line in stay-green pakchoi:1.The male sterile lines’qi 2’and’019’were used as sterile sources,and’CX010’and’201607178’were used as target backsterile lines.After 3 generations of continuous backcrossing and testing,the retention and dual-use lines consistent with the target strain traits were created,and’GMS_CX010’and’GMS₁₇₈’with 100%sterile strain rate and 100%sterile lines were prepared.2.With Shanghai green stem dish’618’as the sterile source and DH line as the target strain,’CMS₀₀₂’,’CMS₀₀₅’,’CMS₀₁₃’,’CMS₀₂₁’,’CMS₀₃₉’were cytoplasmic male sterility lines,with 100%sterility rate and 100%infertility.3.’CMS₀₀₂’,’CMS₀₀₅’,’CMS₀₁₃’and’CMS₀₂₁’were crossed with several excellent inbred lines,and’618’,’701’and’Xia Di’were selected,and the green hybrid’ZH22’and heat-resistant hybrid’005C₃×D344’、’004C₃×D346’、’005C₃×D346’、’004C₃×D348’and’005C₃×D348’were selected.