Effect Of Morus Alba Extract On Production Performance,Egg Quality,Plasma Antioxidant Capability And Biochemical Parameters Of Laying Hens

Mulberry leaf extract(MLE)contains flavonoids,polysaccharides,alkaloids and other physiologically active substances.Numerous studies have shown that Mulberry leaf extract has physiological effects such as antioxidant,hypoglycemic,hypolipidemic,antibacterial and anti-inflammatory.The aim of this study was to investigate the effects of mulberry leaf extract on the production performance,egg quality,plasma antioxidant and serum biochemical parameters of laying hens.A total of 600 black-feathered green-shelled laying hens at 22 weeks of age were selected and randomly divided into 4 groups of 6 replicates of25 birds each,and the trial was divided into the early(1-7 weeks)and late(8-14 weeks)periods.The pre-testing period was 1 week and the main trial period was 14 weeks.The maize-soybean meal type diet was supplemented with 100 mg/kg,200 mg/kg and400 mg/kg of mulberry leaf extract on top of the basal diet.The main trial results were as follows.(1)Compared with the control group,there was no significant difference in the average egg weight,deformed egg rate and dead scouring rate of laying hens in the experimental groups(P>0.05).The average early feed intake of 400 mg/kg mulberry leaf extract layers was significantly reduced(P<0.05);the average feed intake of 200 mg/kg and 400 mg/kg mulberry leaf extract layers decreased significantly during the whole period(P<0.05));100mg/kg and 400 mg/kg mulberry leaf extract layers significantly reduced the feed-to-egg ratio in the late stage(P<0.05).The results show that 400 mg/kg mulberry leaf extract can reduce the average feed intake and feed-to-egg ratio of laying hens,which is beneficial to improve the production performance of laying hens.(2)Compared with the control group,there was no significant difference in eggshell strength and thickness,egg shape index,egg yolk index,egg yolk color,relative weight of egg yolk and egg shell and number of eggs with spots in the test groups(P>0.05).The Hastelloy unit of the 400 mg/kg mulberry leaf extract group was significantly increased by8.51%(P<0.05).Mulberry leaf extract did not significantly affect the total cholesterol,triglycerides,crude protein and crude fat content of eggs(P>0.05).The results show that 400mg/kg mulberry leaf extract can increase the Hastelloy unit of eggs.(3)Compared with the control group,the plasma SOD,CAT and MDA of the laying hens in the test groups were not significantly different(P>0.05).The 200 mg/kg and 400mg/kg mulberry leaf extract groups significantly increased the plasma T-AOC content of laying hens(P<0.01);the 100 mg/kg and 200 mg/kg mulberry leaf extract groups significantly increased the plasma GSH-PX content of laying hens(P<0.01).The results show that the addition of mulberry leaf extract to the basal diet improves the antioxidant capacity of laying hens,which is of great significance to alleviate the antioxidant stress,thereby protecting the health of the body.(4)Compared with the control group,the plasma TP,ALT,AST,TG,TC,CREA,UREA,HDL and LDL of the experimental groups were not significantly different(P>0.05).The 200mg/kg and 400 mg/kg mulberry leaf extract groups significantly reduced the plasma GLU content of laying hens(P<0.05).The results show that the mulberry leaf extract can reduce the blood sugar of laying hens to a certain extent,and has no adverse effects on the liver,kidneys and metabolism in the body.In summary,the addition of mulberry leaf extract to the diet can reduce the feed intake and the feed-to-egg ratio of the layer,increase the Hastelloy unit,increase the content of T-AOC and GSH-PX in the plasma of the layer,and reduce blood sugar,thereby saving feed and saving Feeding costs can improve the antioxidant performance of laying hens and can regulate sugar metabolism.In this experiment,the dosage of 400 mg/kg is the best.