The Evolution Analysis And Function Prediction Of Paulownia WPR Gene Family

Paulownia is a deciduous trees,belong to Paulownia genus,native to China,which was widely planted and cultivated for a long time.The crown shape is beautiful,the trunk is straight,the crown is big root system is deep,huge economic and ecological value.Paulownia witches’ mnbroom is the main reason to limit the development of Paulownia industry.In the process of paulownia growth,it is easy to be infected by the phytogen carried by insects,resulting in paulownia arbuscular disease,resulting in axillary buds,leaf yellowing,leaf shrinkage,internodes shortening.Our previous study found that the Pawb18 was the phytogen effector of Paulownia arbuscular disease.The interaction protein annotated as Plastid Movement Impaired 2(PMI2)was obtained by double screening of Pawb18,and the interaction between them was verified by BI-FC、CO-IP and other experiments.Transcriptome data have also confirmed that PMI2 genes are up-regulated during the pathogenesis of paulownia arbuscular disease,suggesting that it is highly likely to be associated with paulownia arbuscular disease.PMI2 as a member of the WEB1/PMI2-related(WPR)family and a member of the family Weak Chloroplast Movement under Blue Light1(WEB1)has been demonstrated in arabidopsis to be an essential gene for regulating chloroplast light avoidance responses under strong light,thus participating in the regulation of leaf yellowing phenotypes.Therefore,the genetic identification,phylogenetic tree study,collinearity analysis,physical and chemical properties and secondary structure prediction,gene structure motif analysis and functional prediction with the help of transcriptome and interaction protein in the WPR gene family of Paulownia in this study are helpful for us to fully understand the properties of Paulownia WPR gene family and its function during the occurrence of Paulownia arbuscular disease.It provides a basis for further understanding the mechanism of Paulownia arbuscular disease.The results of this paper are classified as follows:1、The herbaceous Arabidopsis,Rice,Maize,woody plant Jujube,Vitis vinifera,Hevea brasiliensis and Populus trichocarpa were analyzed.Based on paulownia genome data,a total of 22 undetermined members were obtained by local BLAST and HEMMER software.After conservative domain prediction,6 members without WEMBL conserved domain were excluded and 16 paulownia WPR family members were identified.2、Through phylogenetic tree analysis,Paulownia WPR family members can be divided into four subfamilies.Analysis of Paulownia WPR gene selection pressure showed that selective pressure resulted in a large proportion of non-synonymous substitution and synonymous substitution in evolution.3、Paulownia WPR family members may have multiple replication and homologous exchange events during evolution.The results of genetic collinearity analysis showed that except PfWPRb3/PfWPRb4 、 PfWPRa2/PfWPRa3 there was single collinearity among subfamilies,PfWEB2/PfWPRb1 there was single collinearity among subfamilies,and other genes had multiple interfamily collinearity.4、The analysis of gene structure found that among the eight family members of the PfWEB、PfWPRb subfamily PfWEB1、PfWEB2、PfWPRb4 the three members had the phenomenon of inclusion deletion,while among the eight family members of the PfPMI2、PfWPRa subfamily PfPMI5、PfWPRa2、PfWPRa3 the three members had the phenomenon of UTR loss.5、Predicting the function of paulownia WPR family genes in the pathogenesis of paulownia arbuscular disease.According to sequence alignment and cis-acting element analysis,Paulownia WPR gene can regulate chloroplast movement,and then regulate leaf photosynthesis and leaf color function.By simulating the pathogenesis of arbuscular disease by seedling and reagent treatment,it is inferred that the PfWPRa2 is low expressed in the pathogenesis process,which leads to leaf yellowing.The interaction between PfWEB3 and PfWPRb3、PfWPRb4 was predicted by interaction protein,which resulted in leaf yellowing.