| Samia ricini,Bombyx mori and Antheraea pernyi are silk insects with important economic value in China.Samia ricini nucleopolyhedrovirus(SariNPV)is one of the main pathogens of Samia ricini sericulture and its infection causes severe impacts on economic sericulture development.In this research,the expression profiles at gene and protein level in the midgut of Samia ricini B7 after infected with SariNPV were analyzed by transcriptomics and proteomics.The research results will provide theoretical guidance for the breeding of resistant varieties.The main research results are as follows:(1)High-throughput sequencing of Samia ricini B7 midgut tissue before and after feeding SariNPV was performed by RNA-seq technology,and differentially expressed genes(DEGs)were statistically analyzed.A total of 2 535 DEGs were identified,of which 1264 DEGs were up-regulated and 1 271 DEGs were down-regulated.GO enrichment analysis showed that 2 479 differential genes were annotated into 683 GO terms,and KEGG pathway enrichment analysis showed that 776 DEGs were enriched in 111 pathways.The GO items and KEGG pathway involved indicate that Samia ricini’s infection with SariNPV may cause damage to organelles,affect enzymatic operation and energy metabolism,lead to obstruction of biosynthesis and nutrient absorption,damage the immune system,and inhibit the growth and development of various systems in Samia ricini’s body.(2)Proteomic analysis of Samia ricini B7 midgut tissue before and after feeding SariNPV using i TRAQ technology identified a total of 434 DEPs,of which 339 DEPs were up-regulated and 95 DEPs were down-regulated.The GO enrichment analysis showed that313 DEPs were enriched in the GO term,and the KEGG pathway enrichment analysis showed that 218 DEPs were enriched in the KEGG pathway.The GO entries and KEGG pathways involved indicate that: after SariNPV infected Samia ricini,the relevant metabolic pathways were blocked,affecting the normal metabolism of the body,resulting in disorder of cell function and change of enzyme activity.Meanwhile,JAK-STAT and other immune pathways were activated to respond to the invasion of the virus.(3)Through the combined analysis of transcriptomics and proteomics in the midgut of Samia ricini,159 DGEs/DEPs were identified.The Wnt signaling pathway,JAK-STAT signaling pathway,Toll and Imd signaling pathway are related to cytopathic changes,indicating that these immune pathways are closely related to resist SariNPV invasion.In this research,we aim to reveal the molecular mechanism of pathogen–host interactions in SariNPV-infected Samia ricini through transcriptomic and proteomic analyses.Using RNA-sequencing and i TRAQ,we mapped the DEGs and DEPs that are involved in the immune responses of Samia ricini upon virus invasion.We identified specific DEGs and DEPs that are involved in various essential biological signaling pathways and immune-related pathways upon SariNPV invasion.These DEGs and DEPs play an important role in triggering host immune responses to pathogens.Not only provide insights into the pathogenic mechanism of SariNPV infection in Samia ricini and the immune response mechanism within the host,but also provide a reference for identifying and preventing diseases caused by SariNPV.In particular,the immune response mechanism of Samia ricini in response to SariNPV infection,and provide a foundation for the early prevention and molecular detection of SariNPV. |
