The Correlation Research Between PLAG1 & LTBP2 Gene Mutations And Thoracolumbar Vertebrae Number And Physical Morphology Of Dezhou Donkey

Equus asinus belongs to Perssodactyla.Local donkey breeds in China are rich in resources and have good stress resistance.Their meat and skin production performance are not prominent now so it is of great significance to cultivate new strains with outstanding meat production and skin yield performance and suitable for large-scale breeding on the basis of local donkey breeds.The preliminary slaughter test of the research group found that Dezhou donkey also has thoracolumbar variation performance.The increase of the number of thoracolumbar vertebrae will lead to longer waist and abdomen of livestock,and then the increase of meat production and skin yield.Therefore,the purpose of this paper is as follows(1)The distribution of thoracolumbar number in Dezhou donkey population and the changes of body size and slaughter characteristics caused by the variation of thoracolumbar number;(2)Developing a method for detecting the number of thoracolumbar vertebrae of living animals in vitro;(3)The correlation between PLAG1 and LTBP2 gene polymorphisms and thoracolumbar number,body size and carcass traits of Dezhou donkey.In this paper,650 adult Dezhou donkeys were collected from Dezhou donkey farms in Yucheng City,Dong’e County and Chiping County,Shandong Province.Among them,486 samples were slaughtered(with slaughtering properties and blood samples),and the number of thoracolumbar vertebrae of the remaining 164 samples were tested in vitro.All samples were used to analyze the distribution of the number of thoracolumbar vertebrae in Dezhou donkey population and the effect of different thoracolumbar vertebrae on body size and carcass traits.395 Dezhou donkey samples with complete phenotypic data were genotyped for the polymorphic loci of PLAG1 and LTBP2 genes,and these loci were used to construct the haplotypes.The association analysis were conducted between the genotype and haplotype of PLAG1 and LTBP2 and the thoracolumbar vertebrae number,body size and carcass traits.The results are as follows:The investigation on body size data,thoracolumbar number and carcass traits of Dezhou donkey population.Dezhou donkey has five types of thoracolumbar number combination(T17L5,2.6%;T17L6,10.8%;T18L5,74.6%;T18L6,10.6%;T19L5,1.4%).With one additional thoracolumbar vertebrae,TLL increases by 5.06 cm,body length increases by 1.97 cm,CW increases by 8.88 kg,on average.The T18L6 and T19L5 thoracolumbar vertebrate type of the total number of 24 thoracolumbar vertebrae is the best thoracolumbar vertebrae type.Customization of an instrument for detecting the number of thoracolumbar vertebrae of Dezhou donkey in vitro.Based on Dezhou donkey’s body size data and behavior characteristics,the first mobile Direct Digit Radiography system was customized.The number of thoracolumbar vertebrae of Dezhou donkey in different growth stages can be accurately detected,and the donkey with 24 thoracolumbar vertebrae can be selected as seed.Therefore,the purpose of " Directional cultivation of new strains of Dezhou donkey with multiple thoracolumbar numbers " can be achieved.The association analysis between PLAG1 gene polymorphism and thoracolumbar number,body size data and carcass traits of Dezhou donkey.The PLAG1 gene was deeply sequenced by GBTS liquid chip to accurately detect the variation sites in the whole gene range.In PLAG1 gene,c.1-2627G>A was significantly correlated with body height,individuals with GA genotype population had significantly higher body height than GG genotype.The association analysis between LTBP2 gene polymorphism and thoracolumbar number,body size data and carcass traits of Dezhou donkey.Among the 15 mutation sites we used for analysis,5 SNVs were significantly correlated with TN,12 SNVs were significantly correlated with TL,1 SNV was significantly correlated with STL,12 SNVs were significantly correlated with LN,1 SNVs was significantly correlated with LL,12 SNVs were significantly correlated with SLL,11 SNVs were significantly correlated with TLN,12 SNVs were significantly correlated with TLL,10 SNVs were significantly correlated with STLL(P < 0.05),and multiple sites were significantly correlated with multiple traits.c.3410C>T and c.813-5759G>A were harmful mutations for all traits;c.1671+5985G>C is a harmful mutation for LN,but a beneficial mutation for other traits;c.1671+7183C>T,c.1568+570G>T,c.52176C>A,c.1568+102A>C,c.1281+444G>A,c.813-2755A>C,c.813-4077C>A,c.813-7895A>G,c.813-8026C>T,c.813-9280T>C and c.813-9553T>G are beneficial mutations for LN but harmful mutations for other traits.