| The Epinephelus spp belongs to the family Pinidae and is distributed in the coastal areas near the bottom of the warm waters of various countries around the world.It is a large and precious fish.Because of its rich nutritional value,it is loved by people all over the world.However,due to the high price,it has a high aquatic value.This makes it harvested and farmed in many countries around the world.Nowadays,most of the Epinephelus spp on the market are artificially cultured,and there are still strict requirements for artificial breeding.At this stage,the methods of artificial culture of Epinephelus spp are mainly based on cage culture and pond culture.Some outstanding problems seriously restrict the rapid development of Epinephelus spp aquaculture.The co-cultivation of Epinephelus spp and two kinds of macroalgae(Cladophora spp or Enteromorpha prolifera)was used as the research object to explore the absorption effect of algae on ammonia nitrogen in Epinephelus spp under different temperature conditions.The use of algae can absorb ammonia nitrogen and improve water quality.Based on the balance of ammonia nitrogen release and absorption in the co-culture of fish and algae,the water quality can be improved,and a new scientific and sustainable marine aquaculture system with high efficiency and color filtration is established.The co-cultivation study of Epinephelus spp and two kinds of algae shows that: under the condition of temperature of 10-15℃,1-2g of Cladophora spp or Enteromorpha prolifera can absorb most of the ammonia nitrogen produced by about 200 g of Epinephelus spp larvae,especially in 24 h.Compared with the water body of Epinephelus spp without algae co-culture,the effect is excellent.When 4g of Cladophora spp or Enteromorpha prolifera is added,it can completely absorb ±200g of ammonia nitrogen produced by Epinephelus spp,and it has been continuously consumed.By comparing the ammonia nitrogen content of water at different temperatures for 36 hours,it was found that the higher the temperature,the higher the ammonia nitrogen content in the Epinephelus spp culture water,and the more significant the absorption effect of algae was.CO2-assisted live fish transportation is a new technology that keeps live fish in a sleep state for a long time to reduce transportation costs.By exploring and studying the changes of basic indicators of Epinephelus spp after carbon dioxide anesthesia dormancy.Under carbon dioxide dormancy,when the temperature is 15℃ and the p H value is around 5.4,the Epinephelus spp can completely appear dormant.When the temperature is 25℃ and the p H value is around 5.6,the Epinephelus spp can be completely dormant.It shows that when the temperature is 15℃,under the dissolved oxygen state of 5-10mg/L,when the p H value reaches 5.4,the Epinephelus spp can go dormant.When the temperature is 25℃,under the dissolved oxygen state of 5-10mg/L,When the p H reaches 5.6,the Epinephelus spp can go dormant.The change of CO2 concentration is a direct factor affecting fish dormancy.Under different temperatures and different dissolved oxygen states,the concentration of CO2 in fish dormancy is also different.The higher the temperature,the higher the concentration of CO2 in the water,and the longer the dormancy time.By constructing an artificial seawater circulating inland aquaculture system in which water purification and aquaculture units are connected in series,this system has lower energy consumption compared to other industrial aquaculture systems.The system uses a circulating potential energy to complete the work cycle of the entire system.Reduce floor space,simple system operation,convenient maintenance,low failure rate and low investment.Using a combination of biological and physical filtration technology,the removal efficiency is high.And it is not easy to be affected and restricted by natural environment such as climate and water quality changes.It is convenient to manage fry,can effectively isolate diseases and control the invasion of pathogens,and reduce the incidence of fry.At the same time,we designed and developed two kinds of filtration and sewage suction devices for water purification,so as to further improve some substantive problems in the industrial inland aquaculture system,and fundamentally solve the problem of suspended solids in the water body,Disposal of harmful substances such as particulate matter,fish excrement and bait residues.Provide reliable technical support for industrial farming such as Epinephelus spp. |