Effect Of Maternal Leucine Supplementation During Late Pregnancy On The Energy Supply For Protein Deposition In Offspring Newborn Piglets

In this experiment,200 sows were randomly divided into 4 groups.On the 70 th day of pregnancy,they were fed with basal diet and treatment group supplemented with 0.40%,0.80% and 1.20% leucine respectively.The blood of pigs and newborn piglets was analyzed for blood lipid and blood glucose levels to explore the effect of leucine on maternal-fetal energy substrate transport;the longest dorsal muscle of newborn piglets was collected to detect energy bases such as triglycerides and glucose Substances and mitochondrial energy supply related genes as well as glucose and fatty acid transport,synthesis and energy related genes,to explore the influence and regulation mechanism of energy metabolism related substrate transport,distribution and synthesis,and finally reveal the sow diet in late pregnancy Effects of adding leucine on muscle energy metabolism of piglets.The experimental results are as follows:1.Effect of leucine supplementation in the diet of late pregnancy on blood glucose and lipid levels of sows(1)On the 84 th day of pregnancy,HDL-C content in the blood of sows in the 0.40%-0.80% Leu group increased significantly(P < 0.01).(2)On the 98 th day of pregnancy,the sow plasma TG content in the 0.40% Leu group was lower than that in the control group,the difference was significant(P < 0.01),at the same time,sow plasma TC content was significantly higher than the control group(P < 0.05).(3)On the 110 th day of pregnancy,the plasma TG content of the 0.40%-1.20% Leu group was lower than that of the control group,the difference was significant(P < 0.01);the blood glucose level of 0.40% Leu group was significantly higher than that of control group(P < 0.05).2.Effect of leucine supplementation on energy substrate content in longissimus dorsi muscle of newborn piglets in late pregnancyIn longissimus dorsi muscle of newborn piglets,the serum TG content in 0.40%-1.20% Leu group was significantly lower than the control group(P < 0.01).Effects of dietary supplementation with leucine in late pregnancy on mitochondrial energy supply,glucose and fatty acid transport,synthesis and energy supply in newborn piglets’ dorsal longest muscle(1)After adding 0.80% Leu,the mRNA expression of PGC-1 α and sirt-1 was significantly increased.WB results showed when the amount of leucine is 0.40%-1.20%,the protein expression of p-AMPK is significantly reduced(P<0.05),and the difference between 0.80%-1.20% and 0.40% was also very significant(P<0.05).(2)After 0.80% Leu was added to the diet,the mRNA expression of FATP4 and VLDLR increased significantly(P<0.05),The expression of CD36 mRNA in the dorsal longest muscle of 0.40% Leu group was lower than that of the control group,the difference was significant(P<0.05).(3)When 0.40% leucine was added to the diet,the mRNA expression of ACSL1,FABP3 and CPT-1 increased significantly(P<0.05).In 0.40% Leu group,the mRNA expression of ACSL6 was significantly lower than that the control group(P<0.05).(4)After adding 0.40% leucine,the mRNA expression of SREBP1,PPAR α and PPAR γ decreased significantly(P<0.05).(5)The 0.40% Leu group can significantly increase the mRNA expression of ACACA and GPAM.The results showed that 0.40%-0.80% leucine can significantly increase the expression of FASN protein in the longissimus dorsi of newborn piglets(P<0.05).(6)The mRNA expression of glucose transporters GLUT1,GLUT4 and SGLT3 in the 0.40% Leu group showed an upward trend,but there was no significant difference from the control group.WB results show that the addition of 0.80% leucine to the sow diet in the late pregnancy can significantly increase the protein expression of GLUT1 in the piglet’s longest muscle(P<0.05).(7)In 0.40% leucine group,the mRNA expression of PEPCK that glucose and glycogen synthesis related gene was significantly higher than the control group(P<0.05).(8)when the amount of leucine added reached 0.80%,the mRNA expression of ACL in the dorsal longest muscle was higher than that of the control The group rose significantly(P<0.05).In summary,adding 0.4% to 0.8% leucine to the diet of pregnant sows can promote protein deposition in newborn piglets,and the energy required for protein synthesis may come from the activation energy supply of fatty acids and glucose.In this process,leucine can reduce the intake of fatty acids in the longissimus dorsi of newborn piglets,increase fatty acid synthesis and intracellular transport,promote fatty acid activation and increase energy sources.This may be the addition of light to the diet in the late pregnancy.Acid promotes one of the important mechanisms of proteinsynthesis in newborn piglets.