Molecular Mechanism Of Potato StPAP10b In Response To Low Phosphorus Stress

Potato(Solanum tuberosum L.)is one of the major grain crops in China.Phosphorus as an essential nutrient element has a great influence on potato plant growth and development.Acid phosphatase is an acid hydrolase containing binuclear metals,which plays an important role in plant adaptation to phosphorus deficiency stress.Until now,the molecular mechanism of potato adaptation to phosphorus deficiency stress is unclear.Whether acid phosphatase related gene exists in potato,and its function at phosphorus deficiency stress needs to be studied.In this research,PAP genes in potato were screened and functional identified.Furthermore,through genetic transformation and phenotypic identification,the effect of acid phosphatase gene St PAP10 b on potato response to low phosphorus stress was studied.The main results were as follows:1.Sixteen candidate PAP genes were found through transcriptome analysis,and the expression differences of these genes under low phosphorus stress were further verified by fluorescence quantitative PCR.Phylogenetic tree analysis showed that St PAP10 b was close to Arabidopsis thaliana At PAP10 and soybean Gm PAP10.Sequence alignment results showed that St PAP10 b had five conserved domains and seven invariant amino acid residues.2.Real-time PCR was performed on different tissues of phosphorus deficiency treated potatoes,and the results showed that St PAP10 b was a root-specific gene.Besides,St PAP10 b was identified that specifically sensitive to phosphorus deficiency stress.3.Overexpression of St PAP10 b in potato showed that the root length,fresh root weight and total phosphorus content in transgenic potato was better as compared with that of wild type under phosphorus deficiency condition.4.Further phenotype investigation in overexpression lines and wild type showed St PAP10 b overexpression lines could utilize extracellular d NTP to anti-phosphorus deficiency effects.5.The subcellular localization of St PAP10 b was observed by stable transformation in potato,and the results showed that St PAP10 b was localized in the cell wall.To sum up,this study revealed that St PAP10 b can response to phosphorus deficiency stress,and elucidated the molecular mechanism of St PAP10 b involved potato resist to the phosphorus deficiency.These results will give us a theoretical basis for potato scientific cultivation and breeding in phosphorus deficiency condition.