| The purpose of this study was to investigate the effects of tryptophan and indoles on the intestinal amino acid transporter and intestinal microorganism of 1-21 days old Magang goose.A total of 240 healthy 1-day-old Magang geese were randomly divided into 4 treatments,each treatment was repeated 6 times,each 10 times.The experiment used single factor experiment design with four groups,CON,Trp,IAA,IPA,and the dietary tryptophan,indole-3-acetic acid,and indole-3-propionic acid are added in an amount of 0.1%The results show:(1)Under the conditions of this experiment,supplement with 0.1% tryptophan significantly reduced the average daily weight gain compared with other groups(P<0.05),and had no significant effect on other indicators(P >0.05).(2)Under the conditions of this experiment,supplement with tryptophan and indoles had no significant effect on serum biochemical indicators.The residual results of the serum indicated that the treatment was effective(P<0.01),and the content of picolinic acid in tryptophan group was higher than other treatment groups(P <0.05).No significant effect on liver antioxidant(P >0.05).(3)Under the conditions of this experiment,Compared with the indole-3-acetic acid and indole-3-propionic acid groups,the tryptophan group significantly reduced the ratio of duodenal villus height and crypt depth(P <0.05).The ratio of duodenal villus height to crypt depth was significantly increased in the indole-3-propionic acid group,the control group,and the tryptophan group(P <0.05).The addition of indole-3-propionic acid and tryptophan significantly increased the expression of SLC6A19 and ACE2 in the jejunum(P <0.05),compared with the control group.The addition of indole-3-propionic acid decreased the expression of SLC6A19 and ACE2 in the ileum compared with tryptophan group.The effect of tryptophan on amino acid transport was mainly through the TOR pathway.(4)Under the conditions of this experiment,supplement with tryptophan and indoles can increase the number of OTUs,and the tryptophan group can increase the richness of the microbial community(P <0.05).The addition of indole-3-acetic acid increased the relative abundance of Bacteroides at the gate level(P <0.05).Compared with the control group,the treatment groups reduced the relative abundance of Megamonas at the genus level(P <0.05)and the relative abundance of Megamonas at the species level(P <0.05).(5)Under the conditions of this experiment,AHR and TOR have a significant effect on the Firmicutes and are positively correlated,TPH1 is negatively correlated with Epsilonbacteraota.Barnesiella has a significant effect on AHR,Campylobacter and TPH1 show negative correlation,Erysipelatoclostridium has a significant effect on AHR and S6K1,Romboutsia has a significant effect on SLC6A19,Shuttleworthia has a significant effect on S6K1,and Turicibacter has a very significant effect on SLC6A19 and ACE2.(6)Under the conditions of this experiment,differences in the metabolic pathways of KEGG indicate that the addition of tryptophan and indole has a significant effect on the ribosomal pathway in translation during genetic information processing,and affects signal transduction,membrane transport and genetic information pathway.In summary: After supplement with tryptophan and indoles,tryptophan and indole-3-propionic acidcan promote amino acid transport,and tryptophan may function through TOR.Tryptophan already indole can increase the number of intestinal microorganisms and change the diversity of microorganisms,and the changes in amino acid transport may be related to intestinal microorganisms. |
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