| With the development of urban greening and the continuous improvement of people’s requirements on environmental quality,more and more garden waste composting is an important way of resource utilization,which contains a large amount of lignocellulose difficult to degrade,which seriously restricted composting treatment of garden waste.This study focused on the construction of high-efficiency cellulose-degrading microflora and the selection of suitable adsorption carriers in the compound microbial agents,and applied it to the treatment of garden waste composting,to study the impact on the physical and chemical properties of the composting materials.The purpose of this study is to improve the efficiency and quality of garden waste composting and to provide theoretical basis and possible application approaches for the utilization of garden waste resources.The results of the study are as following.(1)Promicromonospora thailandica(A),Burkholderia fungorum(B),Anreobasidium pullulans(C),Aspergillus fumigatus(D)and Bacillus mojavensis(E)grew better in PDA culture medium with initial pH of 7.08～7.63 than beef extract peptone and nutrient agar.The bacteriostatic experiment showed that the cellulose-degrading microflora was constructed with the ratio of A:B:C:D:E=9:6:3:4:4 could significantly reduce the antagonism among the 5 strains.The degradation experiment of filter paper strips showed that the microflora constructed with this ratio had good cellulosic degradation effect.(2)Five kinds of carriers,including bagasse,straw powder,vermiculite,diatomite and activated carbon,were selected to study the adsorption of microorganisms,water absorption,effective bacteria release number and proportion.It was found that diatomite had the best adsorption effect and water absorption among the 5 carriers,and was a suitable carrier for high-efficiency cellulose-degrading microflora.(3)Three treatments were set:adding BCE mixed bacterial fluid,adding ABCDE mixed bacterial fluid in proportion of the experiment,and adding complex microbial agents mixing ABCDE in proportion of the experiment with diatomite carrier.It was found that adding high-efficiency cellulose-degrading microflora could aggravate the microbial activities,increase the temperature and pH value,and decrease the EC value,C/N value and cellulose content in the pile material through their influence on the physical and chemical properties of garden waste;the compound microbial agent made of high efficiency cellulose-degrading microflora mixed with diatomite can make the compost more thoroughly degraded and improve the compost’s maturity.After the addition of BCE mixed bacteria solution,ABCDE mixed bacteria solution and ABCDE combined diatomite carrier,it was found that the addition of ABCDE high cellulose-degrading microflora could increase the microbial activities in the composting material,increase the temperature and pH value,and decrease the EC value,C/N value and cellulose content.The compound microbial agent made of high-efficiency cellulosedegrading microflora mixed with diatomite can make the composting more thoroughly and reach the composting ripeness faster. |
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