Analysis Of Potato CNGC Gene Family And Preliminary Verification Of Its Member StDND1 Function

Potato is one of the most important food crops in the world.The major potato producers are China,Russia,India,Ukraine,and the United States,among which China is the country with the largest total potato production in the world.Late blight is one of the most serious diseases in potato production.It not only increases the capital investment in potato production,but also reduces the quality and yield of potato,which seriously restricts the development of potato industry.Previous studies showed that silencing the DND1 homologue gene in potato can improve potato resistance to late blight,but the mechanism of resistance is still unclear.DND1 is an important member of the CNGCs family,so this study identified the members of the CNGCs family in potato on the basis of previous work,analyzed the expression changes of different members under different stresses,analyzed the physiological changes of potato leaf silenced with StDND1,and selected the proteins interacting with this gene preliminarily by using yeast two hybrid.The main research results are as follows:1.A total of 11 StCNGCs were identified in the potato genome,and all the 11 StCNGCs had complete CNBD and PBC domains.A number of cis-acting elements related to phytohormone signalling were predicted upstream of StCNGC ORFs.The expression analysis of StCNGCs showed that StCNGCs were involved in plant growth and stress response.Among them,StCNGC18 can be induced high expression under both abiotic stress and biological stress.2.The silencing of StDND1 gene resulted in less cellulose,hemicellulose,and pectin contents in potato leaves,but there was no significant change in leaf thickness.The silencing of the StDND1 gene leads to an increase in the number and volume of the wax layer crystals in the epidermis of potato leaves,and a significant increase in the wrinkles on the leaf surface,but the overall thickness of the wax layer did not change significantly.The silencing of StDND1 gene resulted in a significant increase in relative electrical conductivity and a significant decrease in chlorophyll content of plants.It was also speculated that the silencing of StDND1 gene led to the premature senescence of potato.3.The yeast two hybrid text library constructed by using StDND1 as bait protein filtered out 17 proteins that may interact with StDND1.At present,none of these 17 genes has been reported to interact with StDND1.These results suggested that several members of the StCNGCs family were involved in stress,and StDND1 may not only improve potato resistance to late blight by affecting the changes of cell wall and other substances,but also realize potato resistance to late blight by interacting with various proteins.Therefore,the rational utilization of susceptible genes will provide new ideas and approaches for improving the disease resistance of plants.