Functional Analysis Of The Gene Family Of GET Pathway Orthologs In Magnaporthe Oryzae

Rice blast,which caused by hemi-biotrophic ascomycete fungus Magnaporthe oryzae is the most destructive disease of cultivated rice,and seriously threaten rice production and global food security.The GET complex is responsible for insertion of secretory pathway tail anchoring proteins(TA)proteins into the ER membrane and represents a critical mechanism for ensuring efficient and accurate targeting of TA proteins.Although the GET complex play a role in yeast,Arabidopsis thaliana and mammals,the function of GET complex are different among thouse three.And its function in Magnaporthe oryzae has not been reported.In this study,we identified 5 proteins(Get1,Get2,Get3,Get4,and Sgt2)belonging to GET complex in M.oryzae and generated the corresponding gene knock-out mutant.We found that both Mo GET1 and Mo GET2 are required for hyphal growth and pathogenicity;While the loss of Mo GET1,Mo GET4 or Mo SGT2 did not affect the normal development of the rice blast fungus.Protein interaction analysis revealed that Mo Get1,Mo Get2,Mo Get3 could interact with each other in vivo and in vitro.Furthermore,Mo Get1 and Mo Get2 is co-localized on the endoplasmic reticulum(ER).We then generated the Mo GET1 and Mo GET2 double gene knock-out mutant which severely impaired sporulation and pathogenicity and caused hyphal autolysis.Taken together,these results showed that the GET complex is important for hyphal growth,asexual development,and pathogenesis of M.oryzae,and molecular mechanism of pathogenesis requires further investigations.