| Most of the Tangchuan(Camellia sinensis(L.)O.Kuntze)is a natural growth,distributed in different villages of Tangchuan.Because of the specifical geographical,The TangChuan(Camellia sinensis(L.)O.Kuntze)tastes bitter.While the use of resources and breeding of TangChuan(Camellia sinensis(L.)O.Kuntze)is still in the early stages,its taste had not been analyzed.Therefore,it is very important to study its bitter and genetic relationships.1 The optimal method of HPLC for the determination of catechins and alkaloids in teaHPLC is used for the determination of catechins and alkaloids in tea,We analyze the tea extract catechins and alkaloids with retention time and external standard method to spectrum qualitative,The correlation coefficient of regression equation of them is more than 0.9990,there is a good linear relationship between the components,the recovery rate is 103.68%-82.94%,RSD is 0.23%-3.31%.The method is simple,rapid and accurate,which can be used to determine the content of catechins and caffeine in tea.2 The analysis of catechins,alkaloids,amino acid content in Tangchuan(Camellia sinensis(L.)O.Kuntze)In the analysis of Tangchuan(Camellia sinensis(L.)O.Kuntze)of catechin,alkaloids,amino acid.The average content of EGCG is 1.3 times of Fuyun6,the coefficient of variation of ester type catechin content reached above 0.29.the alkaloid content of the average value is higher than fuyun6,the theobromine content of the average value is 1.56 times of Fuyun 6.the caffeine of coefficient of variation is about 0.2;the content of amino acid components are higher than the average value of almost Fuyun6,bitter amino acid,flavor amino acid and sweet amino acid of coefficient of variation was about 0.3.The content of catechins,alkaloids,amino acid has diversity in TangChuan(Camellia sinensis(L.)O.Kuntze).Principal component analysis on the components of catechin,amino acids and alkaloids,the result suggest that the cumulative contribution of the first 8 principal components was up to 81.62%。The first principal component contribution rate is 20.48%,and the corresponding eigenvector is a greater amount of EGCG,catechins,followed by Caf,alkaloids,TB,ECG.EGCG,ester catechins alkaloid,Caf,TB,ECG was positively correlation with the first principal component.When the first principal component has a large portion,which is conducive to the high ester catechin,improving the quality of high EGCG and high alkaloid resources.In the future,the weight of each principal component should be increased or decreased according to the different tea breeding targets in the future.3 Bitter taste evaluation system establishment and the analysis of TangChuan(Camellia sinensis(L.)O.Kuntze)We analysis of 37 trees of TangChuan and Fuyun6 by using the method of sorting and magnitude estimation.The result showed that TangChuan(Camellia sinensis(L.)O.Kuntze)bitter degrees range of 1.65 to 4.74,and the average degree value is 3.05 which is 2.07 times high of FuYun6.In different areas,the bitter taste value of GuangMing village ranges of 2.50 which is the lowest.While the value in ChiMu village(Protected area)is the highest(the value range of 3.16).4 The genetic analysis of TangChuan(Camellia sinensis(L.)O.Kuntze)by ISSR markerWe select 37 trees TangChuan(Camellia sinensis(L.)O.Kuntze)and 1 tree of FuYun6 which is used as contral to UPGMA cluster analysis.According to the Clustering analysis diagram,38 trees plant can be divided into two big class by the Similarity coefficient of 0.54.The first class is FuYun6,and the second branch is TangChuan.When we improve the Similarity Coefficient upto 0.59,the TangChuan can be divided into three branches,the first branch is QiuShan village resourse(named DY14),the second branch is GuangMing village resource(named C1,C2,C3,C4,C5,C6)which is content all the sampling.The third branch is ChiMu village(Non Protected area)respurce E1.When the Similarity coefficient is from 0.71 to 0.73,the other resources of ChiMu village(Non Protected area)content E2,E3,E4,E5,E6,E7 are in the same branch.it is indicated that TangChuan has the regional characteristics.According to the Nei’s genetic similarity coefficient and genetic distance in different regional,the similarity coefficient between ChiMu village(Non Protected area)with QiuShan village is 0.9287,while the genetic distance between the two village is just 0.074,which is reflecting the low degree of genetic differentiation between the two groups. |
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