Functional Analysis Of ZmLOX1 And ZmLOX2 In Zea Mays

Maize(Zea mays)as one of the most important foods in the world,every year produces 0.13 billion tons in China,about 28%of the country’s total grains output.Anthracnose leaf blight and stalk rot diseases are kinds of destructive maize diseases,caused by fungus Colletotrichum graminicola,account for a huge losses in annual corn production.Jasmonic acid(JA)plays multiple and important roles in biotic and abiotic stress response,growth,development and senescence of plants.Till now only a little of JA biosynthesis enzymes have been characterized in maize.This research is mainly about LOX genes in maize family ZmLOX1 and ZmLOX2.according to the characters of ZmLOXl and ZmLOX2,we suspected ZmLOXl and ZmLOX2 are involved in JA biosynthesis pathway against biotic stress and abiotic stress.Reverse genetic method was used to generate the ZmLOXl and ZmLOX1 mutants.The leaves of both mutants are quite sensitive to the C.graminicola infection,however,their stems are more resistant to the pathogen.The mutants are also sensitive to salt stress when comparing to the near-isogenic wild types.The phytohormones of JA,SA and ABA in the mutants were detected,which suggested that ZmLOXl and ZmLOX2 are involved in JA biosynthesis pathway.Our results will help us to better understand the JA biosynthesis pathway in maize and its roles in regulation of biotic stress and abiotic stress in maize.