| Organic solid waste refers to waste consisting of biomass or organic substances,including agricultural waste and food waste,etc.With the acceleration of modernization,the effective disposal of organic solid waste has become an urgent problem to be solved at present.Traditional organic solid waste disposal methods include composting,pyrolysis and anaerobic digestion.Composting disposal takes up a lot of space,takes a long time and has low treatment efficiency;pyrolysis disposal has high energy consumption and high equipment requirements;anaerobic digestion disposal has a long treatment cycle and high equipment costs.Therefore,it is of great significance to solve the problem of organic solid waste treatment and disposal scientifically and rationally,and to realize the harmless,reduction and resourceful treatment of organic solid waste.Microbial fuel cell(MFC)technology is a technology that uses microbial metabolic reactions to directly convert organic matter into electrical energy.As a green,low-cost and sustainable way to obtain energy,MFC technology has received a lot of attention in recent years.The treatment of organic solid waste with MFC technology to produce electricity is expected to be an effective means to solve the organic solid waste disposal problem and energy crisis.However,the large molecules in organic solid waste are often difficult to pass through the cell membrane of microorganisms and need to be degraded into small molecules by the action of extracellular enzymes before they can be absorbed and utilized by microorganisms.Therefore,the hydrolysis stage is also the rate-limiting step of the whole organic solid waste resource utilization.As an efficient and environmentally friendly pretreatment technology,enzymatic hydrolysis technology has been widely used in the field of organic solid waste pretreatment.This paper expands the application of MFC in organic solid waste treatment by constructing a two-phase coupling process based on enzymatic hydrolysis pretreatment and MFC.The technical feasibility of using enzymatic hydrolysis and MFC two-phase coupling for electricity generation from different agricultural wastes(bamboo chips,bacillus rods and rice husk)was studied;the operation modes of hydrolysis pretreatment and hydrolysis product MFC electricity generation from organic solid waste were designed and optimized according to the composition of different organic solid wastes to realize the feasibility study and efficient and stable operation of MFC electricity generation with organic solid waste as the substrate.Meanwhile,the feasibility of the two-phase coupled hydrogen and ethanol production process based on enzymatic hydrolysis pretreatment and anaerobic fermentation of food waste was investigated,and a comparative analysis was conducted with the MFC electricity production process of food waste in terms of efficiency,energy consumption and environmental impact.Finally,a multi-stage neural network model-based MFC power generation system is constructed to provide a scientific and theoretical basis for its subsequent large-scale industrial application.The specific content in this study contains the following aspects:(1)Enzymatic hydrolysis and MFC two-phase coupling technology to treat agricultural waste for electricity production.The hydrolysis effects of different agricultural wastes(mycorrhizal sticks,rice husk and bamboo chips)were investigated and the MFC power production test was performed using the hydrolysate of agricultural wastes(bamboo chips,mycorrhizal sticks and rice husk).Compared with bamboo chips and sticks,the MFC obtained a higher output voltage(0.39 V)and a maximum power density of 26.02 m W/m~2 when treating rice husk hydrolysate,showing a relatively stable power production performance(single power production cycle higher than 300 h).Therefore,rice husk was the optimal power-producing substrate compared to sticks and bamboo waste.(2)Two-phase coupling technology of enzyme hydrolysis and MFC for food waste electricity generation.The effect of fungal solid-state fermentation enzymes on the hydrolysis of food waste was investigated,and the system was able to operate continuously and stably for more than four cycles,and the duration of a single power generation cycle was higher than 300 h.The maximum value of power density reached 30.38 m W/m~2,showing good power generation performance.(3)Comparison of MFC electricity production and anaerobic fermentation technologies for the treatment of food waste.Enzyme hydrolysis and MFC two-phase coupling technology to treat food waste to produce electricity,enzyme hydrolysis and anaerobic digestion two-phase coupling technology to produce hydrogen and ethanol were studied respectively,enzyme hydrolysis and MFC two-phase coupling technology to treat food waste to produce electricity has more obvious advantages in treatment efficiency,energy recovery and environmental impact,and has greater development potential in the future.(4)Optimization of MFC power production performance based on multi-level neural network model.The prediction models of power density of MFC power production were established by BP and PSO-BP intelligent algorithms,and the R~2 of BP neural network and PSO-BP neural network were 0.92304 and 0.95369,respectively,with good linear correlation between the two prediction results and the actual data,and the prediction accuracy using PSO-BP neural network model was higher than that of BP neural network. |