Electrochemiluminescence Molecular Imprinted Biosensors Based On Biomass Carbon And Nano-enzyme Materials For The Detection Of Agricultural Residues

Electrochemiluminescence（ECL）is a new type of electrochemical detection technology.It combines the advantages of spectrum analysis and electrochemical analysis with high sensitivity,simple operation,quick response,and many other advantages,which is widely used in many fields.But ECL analysis also has some limitations,it does not have good specificity recognition ability.Molecular imprinted technology（MIT）is a biomimetic technology that simulates natural enzyme recognition mechanism,and the synthesis of Molecular imprinted polymers（MIP）is relatively simple.Combining MIT with ECL is a good strategy,but the conductivity of MIP is low,which affects the sensitivity of the sensor.In this paper,the ECL intensity was improved by enhancing the electrical conductivity through Biomass carbon（BC）and develop self-enhanced luminescent semiconductor nanomaterials.And then the molecular imprinted electrochemiluminescence sensors were constructed.The research content of this paper is as follows:1.BC/Zn Co MOF@PBA,a co-reaction promoter,was prepared by agitation self-assembly.The doping of BC improved the electrical conductivity and enhanced the catalytic ability of MOF material to the co-reaction agent.and it was also conducive to the formation of MIP through electropolymerization.Using o-aminopenol as functional monomer and dinotefuran as template molecule,MIP was produced by electrochemical polymerization on the modified electrode.Then the MIP was eluted to form imprinted cavity,and a certain concentration of dinotefuran was detected by adsorption.Under optimal conditions,the ECL response of MIP/BC/Zn Co MOF@PBA/GCE to dinotefuran showed a good linearity between 0.01 and 100μmol/L,and the detection limit was0.0046μmol/L,showing a good application for the detection of furtefuran in actual samples.2.Mn₃O₄loaded with Te-Cd S was synthesized by one-pot hydrothermal method as cathode luminophore.Mn₃O₄could catalyze the decomposition of H₂O₂to improve the ECL intensity of cathode.Luminol was added to the buffer solution of the coreactant as the anode luminophore.MIP was polymerized on the surface of the Te-Cd S@Mn₃O₄modified electrode by electropolymerization.When the MIP was eluted,the imprinted cavity was left.The imprinted cavity has a readsorption effect on 2,4-D.When 2,4-D reoccupied these imprinted cavities,the contact between the coreactant and Te-Cd S@Mn₃O₄was blocked as well as the transmission of electrons.The bipolar ECL signal decreased with the increase of the concentration of 2,4-D.The two signals were self-calibrated by the current ratio response of the cathode and anode(I_cathode/I_anode)to improve the accuracy of the sensor.Under optimal conditions,there was a good linear relationship between I_cathode/I_anodeand the concentration of 2,4-D in a certain range,and the detection of 2,4-D in practical samples had a good performance.