Construction Of Surface-enhanced Raman Sensor And Its Application In Chiral Molecular Detection

Chiral recognition is one of the important and special modes of molecular recognition,with wide attention in the fields of biochemistry,pharmaceutical,and drug development.Chirality is a fundamental feature of life processes,and the elements in most organisms,are chiral molecules.Most drugs are also chiral.The performance of the enantiomers of chiral molecules may show significant differences in their biochemical activity,potency,toxicity,transport mechanisms,and metabolic pathways.Diabetes mellitus and its complications have become a worldwide concern,with negative effects on human health.Real-time,convenient glucose detection in biological fluids is urgently needed.Surface-enhanced Raman spectroscopy is a very promising damage-free analysis technology,which can be widely used in biomedical science,food safety,and environmental hygiene,and it is still difficult to identify similar samples using SERS technology.The colorimetric / fluorescence method has the characteristics of high sensitivity,quick reaction,intuition,convenient manufacturing and easy operation.Based on this,a specific enzyme-based multifunctional nanosensing system and a chiral-based organic framework for amino acids are developed in biofluids and medicine.The main research contents are as follows:(1)A three-mode chiral recognition method with fluorescence / colorimetric / SERS was established by using a co-tetrabenethene nuclear thioboric acid as the probe,AuNRs-TPEMBA / GOx-TMB complex,β-D-GOx as the model enzyme,and glucose enantiomer as the substrate.When the glucose isomers are present,Due to the special enzymatic catalysis between D-glucose and β-D-GOx,Leading to TMB in the action of D-glucose with β-D-GOx,Hydrogen peroxide was generated by β-glucose-catalyzed hydrolysis,Hydrogen peroxide causes TMB in the system,Changing the color of the solution from light purple to blue,And gradually deepen;The L-glucose does not cause this phenomenon,However,the diol molecule in L-glucose was closed esterified with the borate molecule of TPEMBA,The new B-O key was generated,And produced a telescopic vibration,Thus,the RIR action,Lead to the aggregation-induced luminescence(AIE)effect to enhance the fluorescence,The characteristic peak of the B-O bond produces,And it increased the intensity.This study established three modes of recognition method,and put forward the new method,this method can well identify the body fluids and chiral glucose,in medicine has a broad development prospect in biomedicine,is expected to be a kind of can detect various chiral sugar in human liquid biomedical added analysis system.(2)Chiral recognition is one of the important and special modes of molecular recognition.We hope to develop a simple,fast,sensitive,and high-throughput method for routine detection of chiral identification.In this study,we demonstrate that metal-organic frame-end-wrapped gold nanorods(AuNR)can serve as a super-efficient platform for enantiomer separation and detection of chiral phenylalanine.The porous properties of the substrate material of AuNR@ZIF-8 showed better selectivity and stability compared to single naked AuNR.The AuNR@ZIF-8/R(FTP)platform accurately identifies D / l-phenylalanine chiral enantiomers by(R)-2-(4-4-propionaldehyde(R-FTP)SERS chiral probes and phenylalanine.This is the first application of a biosensing platform chiral recognition platform based on the nucleotide cap AuNR and opens new opportunities for designing more novel enantiogram sensing strategies and enantiogram-specific adsorbent as well as extending their applications in different fields.