Synthesis And Application Of Iron-Based Metal-Organic Framework Composites And Nano-Copper Oxide

Ferroptosis,as a new modality of regulated cell death,has attracted increasing attention in the field of cancer therapy.Based on the strategy for inducing ferroptosis in tumor cells,we designed and synthesized the iron-based metal-organic framework nanomaterials loaded with simvastatin（SV@MOF）,which were further coated with liposome to form SM@Lip used to mediate ferroptosis in breast cancer cells.The synthetized nanomaterials were characterized by XRD,FTIR,XPS and FE-SEM,of which the results showed that SV@MOF were fusiform in shape,and had high crystallinity with drug loading of 3.75μg/mg.The iron ions mainly existed in the form of Fe²⁺.The SV@MOF were successfully encapsulated with the liposomes to obtain SM@Lip with particle size of approximately 200-300 nm and a coverage rate of14.90%.In vitro cytotoxicity studies indicated that the synthesized SM@Lip had significant inhibitory effects on the growth of the breast cancer cells MCF-7 and MDA-MB-231,and can dramatically increase the intracellular ROS and lipid peroxidation levels.In addition,the results Western blot revealed that the synthesized SM@Lip can up-regulate the expression of the key regulatory proteins p53 of ferroptosis pathways,and simultaneously down-regulate the expression of GPX4,a core regulator in classical ferroptosis,suggested that the SM@Lip can effectively induce ferroptosis in breast cancer cells.Furthermore,the synthesized SM@Lip can be cracked in the tumor microenvironment,and release Fe²⁺and simvastatin,which could get together to play a bidirectional regulatory role in inducing ferroptosis.of those,Fe²⁺can trigger intracellular Fenton reaction,and then increase ROS levels.Simvastatin can act via the mevalonate pathway,down-regulate the expression of GPX4,and decrease the antioxidant capacity.Both Fe²⁺and simvastatin can cause lipid peroxidation and lead to breast cancer cells death by inducing ferroptosis.Green synthesis of nanoparticles based on biological sources have the advantages of being simple,economical,eco-friendly,etc.In this paper,we developed a green synthesis method of copper oxide nanoparticles（CuO NPs）by utilizing the medicinal plant Taraxacum mongolicum extract as reducing and capping agents.The structural properties of the as-prepared CuO NPs were characterized and analyzed by XRD,FTIR,UV-vis,FE-SEM,AFM and EDS.The results showed the synthesized CuO NPs are monoclinic with high crystallinity and purity,and are close to spherical in shape with a particle size of～30-50 nm.The antibacterial assays according to the broth micro-dilution method revealed that the CuO NPs are well capable of inhibiting the activity of Staphylococcus aureus and Escherichia coli.In addition,the performance of CuO NPs for catalytic degradation of organic dyes was also studied.The results showed that the green synthesized CuO NPs can effectively degrade rhodamine B（Rh-B）and malachite green（MG）,especially for MG,which can degrade more than 90%in the period of 210 min.These above-mentioned findings suggest the application potential of the green synthesized CuO NPs in the fields of biomedicine and environmental remediation.