Mechanism Of Dihydromyricetin In Regulating Benign Prostatic Hyperplasia

Benign prostatic hyperplasia(BPH)is a chronic disease that seriously affects the quality of life of middle-aged and elderly men.Histopathologically,BPH is characterized by the irregular proliferation of epithelial cells and fibromuscular tissue in the transitional and peripheral areas of the prostate,resulting in prostatic hypertrophy.Dihydromyricetin,a flavonoid with antibacterial,anti-inflammatory,antiviral,and anticancer activities,is mainly found in the edible and medicinal plant rattan tea.The aim of this study is to evaluate the regulatory effect of dihydromyricetin on BPH and explore its potential mechanism,so as to provide theoretical basis for the development and application of related functional foods.In this experiment,the mechanism of action of dihydromyricetin in regulating BPH was explored through in vivo and in vitro experiments.In vivo experiments,male ICR mice were used to inject testosterone propionate after castration to establish an animal model of BPH,and after treatment with dihydromyricetin,blood biochemical indexes,organ coefficients,histopathological analysis,and protein expression in the organs were detected to determine whether dihydromyricetin was effective in vivo.In vitro experiments by establishing a cell line to explore the mechanism of dihydromyricetin in the treatment of BPH,without affecting the normal prostatic proliferative cell RWPE-1 cells,seeking to inhibit the appropriate concentration of human prostatic proliferative cell BPH-1 cells,and through cytotoxicity test,cell migration experiment,DAPI staining nucleus and other experiments,to observe the effect of dihydromyricetin on cell proliferation,and finally through ELISA,WB experiment,To explore the mechanism of dihydromyricetin in the treatment of BPH in vitro from protein expression levels.The results showed that in in vitro experiments,dihydromyricetin inhibited the proliferation and migration of BPH-1 cells by regulating the levels of androgen receptor pathway proteins(SRD5A2,AR,PSA),apoptosis-associated protein(Bcl-2)and growth factor(p-EGFR).In vivo experiments,dihydromyricetin reduced the prostate organ coefficient,serum hormone level(T,5AR)and expression level of prostate tissue-associated proteins(SRD5A2,Bcl-2,PCNA,VEGF-A,fibronectin)in BPH mice induced bilateral orchiectomy as BPH model mice.In addition,dihydromyricetin significantly improved the histological morphology of the prostate in BPH model mice.In summary,dihydromyricetin improves BPH by regulating androgen signaling pathways and levels of cell proliferation and apoptosis-related proteins.