Preparation Of Copper Selenide And Research On Its Application In Tumor Photothermal Therapy And Chemotherapy

Copper selenide nanomaterials have attracted widespread attention in biosensors,bioimaging,and cancer therapy because they exhibit unique properties in the near-infrared region and can be endowed with various physicochemical properties through chemical modification or doping.However,the application of copper selenide nanomaterials in biomedical fields still needs to be studied and explored in depth.Therefore,in this article,hollow copper selenide nanoparticles（Cu Se NPs）with great biocompatibility were prepared and loaded with the anticancer drug Adriamycin（DOX）to construct a synergistic therapeutic system integrating chemotherapy,photothermal therapy（PTT）,and chemodynamic therapy（CDT）,which exhibited good anti-tumor properties.The specific studies are as follows:1.Hollow Cu Se NPs were prepared by anion exchange method using copper sulfate pentahydrate（Cu SO₄·5H₂O）as the copper source and selenourea（CH₄N₂Se）as the selenium source.The products were characterized by X-ray diffractometer（XRD）,transmission electron microscope（TEM）,X-ray photoelectron spectrometer（XPS）,and other testing means for crystalline phase,morphology,size,and composition.The results showed that hollow Cu Se nanoparticles with uniform particle size were obtained by reacting at 80℃for6 hours,and the particle size was about 50 nm±20 nm.2.The surface modification of Cu Se NPs（Cu Se-PEG NPs）with bis-mercapto polyethylene glycol（HS-PEG-SH）and loading of DOX was used to construct a synergistic treatment system（Cu Se-PEG/DOX NPs）integrating chemotherapy,PTT and CDT.Dynamic light scattering（DLS）tests showed that the Cu Se-PEG NPs were homogeneous and stable,and no significant aggregation after 7 days.The photothermal data showed that the Cu Se-PEG NPs had good absorption in the near-infrared region,with a photothermal conversion efficiency of 54.66%.The electron paramagnetic resonance（EPR）test results showed that Cu Se-PEG NPs could react rapidly with trace H₂O₂ to produce·OH.The UV absorption spectrum data showed that the DOX loading rate of Cu Se-PEG NPs was about 33.7%,which had good drug loading capacity.3.The antitumor properties and biosafety of Cu Se-PEG/DOX NPs were evaluated in vitro and in vivo.In vitro cellular experiments demonstrated that Cu Se-PEG/DOX NPs exhibited good inhibitory effects on mouse breast cancer cells（4T1）,and 95.7%of tumor cells could be eliminated under NIR light irradiation at the sample concentration of 200μg/m L.The in vivo tumor suppression experiments showed that Cu Se-PEG/DOX NPs could effectively eliminate tumor tissues under NIR light radiation,and showed good biosafety with no obvious toxic side effects on the surrounding normal tissues.