Electrochemiluminescence Biosensing Platform For Detection Of Disease Biomarker Based On Multifunctional Nb₂C Mxene

The development of high-performance nanomaterials is of great significance in constructing ECL sensors and improving ECL sensitivity.As an emerging nanomaterial,MXenes have attracted much attention in the field of building biorecognition probe on account of its with ingenious surface properties,tunable band gaps,and excellent photothermal properties,could ccommodate amounts of luminophores and accelerate electron transfer,and.In order to further improve the sensitivity and specificity of ECL analytical technology,it is greatly significantly to broaden the application of MXene in miniaturized ECL sensing.This paper was devoted to the development of novel MXene,exploration of the signal regulation function of MXenes in ECL biosensors,and application of MXene for constructing novel detection platforms,ultimately realizing ultrasensitive detection of cancer markers.The specific research contents are as follows:1.In this work,a novel new ratiometric biosensor based on Nb₂C NS as dual-functional reagent was developed for the first time,which was used for ultra-sensitive detection of hepatitis C virus E2 protein.The new ratiometric biosensor based on a facile nanoprecipitation method through encapsulating Tris（2-phenylpyridinato）iridium（Ir（ppy）₃）into Pluronic block copolymers formed water-soluble F-127@Ir（ppy）₃as the anode emitter and g-C₃N₄as the cathode emitter,where the ratiometric ECL signal could be mediated by using multifunctional Nb₂C nanosheets（Nb₂C NSs）with excellent electrocatalytic activity to enhance the anode ECL strength of and the activity of superoxide dismutase（SOD）,thus quenching the cathode g-C₃N₄ECL response.The combination of F-127@Ir（ppy）₃-g-C₃N₄and signal amplified-Nb₂C NSs not only expand the applification of Nb₂C NSs in ECL but also offer new vista to other biomarker detection.2.A novel self-circulation amplified wireless ECL modular device based on multifunctional conjugated semiconducting polymers as BPE cathode（CFC@P15）was constructed for ultra-sensitivity determination of lung cancer biomarker let-7a.Through target mediated rolling circle amplification（RCA）reaction in BPE cathode,the ECL emitter Ru（phen）₃²⁺and Nb₂C MQDs were introduced into BPE-ECL sensing platform.The self-circulation amplified ECL system was first proposed via P15 as coreactant and photocatalyst roles and Ru（phen）₃²⁺as ECL emitter and light source to realize an efficient and united signal enhancement.Nb₂C MQDs was used as high efficiency probe for furher amplifying the cathodic Ru（phen）₃²⁺ECL response.Compored with traditional ECL sensing platform,the proposed miniaturized BPE-ECL system shows more convenience and gives greatly promising prospects in biomarker of POCT detection.3.Nb₂C MQDs assisted diversified closed bipolar system（d-BPE）for wireless electrochemiluminescence（ECL）signal amplification biosensor was first developed for ultrasensitivity detection of the hepatitis C virus biomarker E2.The d-BPE device was comprised of three series connected ITO electrodes named as ECL reporting channel,HER catalytic channel and recognition channel,respectively;a temperature-sensitive interface was constructed in the recognition compartment of BPE as an electro-conductivity modulator to regulate the current of BPE and obtanined the signal amplication of ECL response.Compared with the traditional photothermal enhancement of ECL signal,this remote manipulation and high precisely invaded a recognition zone reduced the limits of traditional photothermal enhanced ECL signal amplification and achieved the excellent analytical performance of wireless ECL signal.More significantly,the designed d-BPE-ECL configuration greatly extends NIR light driven technology in application in others wireless device.