The Critical Technology And Application On Detection And Distinguish Of Characteristic Volatile Compounds Of Unifloral Honey

Honey is the natural sweet substance produced by bees from the nectar of plants or from secretions of plants or excretions of plant-sucking insects combined with specific substances of their own.According to the number of flower nectar types collected by bees,honey can be divided into monofloral honey and multifloral honey.Each monofloral honey has its unique flavor,which is related to the classes,and concentrations of volatile components.Volatiles of honey is dependent on the botanical source,geographical origin,and honeybee species,as well as the honey quality,processing technology,and storage conditions.Therefore,honey volatiles are of great significance to the study of honey characteristics,the evaluation of honey quality,and the identification of honey authenticity.In this study,two HS-SPME-GC-MS methods were developed to detect volatile compounds and characteristic volatile compounds in monofloral honey in honey based on two chromatographic columns.There are four parts of this research and the main contents are as follows:(1)Based on the DB-5MS chromatographic column,a headspace solid-phase microextraction and gas chromatography-mass spectrometry(HS-SPME-GC-MS)method was developed and validated for simultaneous qualitative and quantitative analysis of 34 volatile components in honey.The volatile fingerprint and quantitative results were simultaneously obtained by using the SCAN+SIM mass spectrometry scanning mode.The limit of quantifications(LOQs)and limit of detections(LODs)of 34 volatile compounds were in the range of 1-10 ng/g and 0.3-3 ng/g,respectively.The spiked recoveries ranged between 70.6% and 126.2%,with the relative standard deviations(RSDs)not higher than45.4%.(2)The developed method was applied to 86 honey samples from six different botanical origins,including linden honey,rape honey,jujube honey,vitex honey,lavender honey and acacia honey.A total of 98 volatile compounds were found with relative contents determined,and the 34 volatile compounds were determined with absolute concentration.Based on the volatile fingerprints and the contents of volatile compounds,honey samples from six botanical origins were well classified by principal component analysis and orthogonal partial least-squares discrimination analysis.(3)Based on the DM-WAX chromatographic column,a headspace solid-phase microextraction and gas chromatography-mass spectrometry(HS-SPME-GC-MS)method was developed and validated for simultaneous qualitative and quantitative analysis of 34 volatile components in honey.The developed method was applied to 86 honey samples from six different botanical origins.