Construction Of Molecularly Imprinted Sensor Based On Nanomaterials And Research On Biochemical Detection

Molecular imprinting technology（MIT）,also called molecular template technology,is a technology that recognizes molecules by self-assembling functional monomers around template molecules through the interaction between functional groups,and is widely used in the fields of molecular and biological detection.Moreover,nanomaterials have the property of simulating enzymes,and have made great progress in the field of catalytic detection.Therefore,by constructing molecularly imprinted sensors,using nanomaterials as carriers,combining molecularly imprinted technology and biological enzymes,the efficient capture and recognition of target molecules can be achieved.The specific work of this paper is as follows:1.Construct a cascade catalytic system through metal oxide biomimetic enzymes and biological enzymes and combine molecular imprinting technology to improve the selectivity of biological enzymes and the detection ability in analogs.Molecularly imprinted polymers（MIPs）were prepared by self-initiated polymerization under mild conditions by continuously generating hydroxyl radicals on the surface of hollow metal cerium oxides（CeO₂HNPs）usingβ-D-glucose as a template molecule.A cascade catalytic system（CeO₂HNPs@GOx-MIPs）with high specificity and selectivity forβ-D-glucose detection was obtained.Impressively,the obtained cascade catalytic sensing system（CeO₂HNPs@GOx-MIPs）exhibited a wide linear range from 1.2μM to 0.8 m M and a low detection limit of 1.0μM.Isomer experiments show that the sensing system has satisfactory selectivity as well as excellent cycle and long-term stability.2.Using metal-organic framework（MOF）to immobilize biological enzymes,and combining biological enzymes and gold nanoparticles with the help of molecularly imprinted polymers to improve the detection ability in biological samples.Using cholesterol as a template molecule,molecularly imprinted polymers were prepared by self-initiated polymerization under mild conditions by embedding gold nanoparticles（Au NPs）on the surface of metal-organic framework（Uio-66-NH₂）and immobilizing cholesterol oxidase（Chox）on the surface.（MIPs）.Impressively,the obtained cascade catalytic sensing system（Uio-66-NH₂@Au NPs-Chox@MIPs）,can be obtained in the range of 2.9 m M-6.7 m M and LOD=2.4 m M by a simple colorimetric method Achieving effective and selective detection of cholesterol in blood.In addition,the sensing system has satisfactory selectivity and excellent stability.