Removement Of Reactive Blue X-BR Dye With Aspergillus/Chlorella Sorokiniana Chitosan Consortium In Photobioreactor

The fungus algae composite system has advantages such as high treatment efficiency,fast startup time,and easy recovery of microalgae in the wastewater treatment process,which has a good application prospect.The fungus chlorella composite system has been proven to be able to remove dyes from wastewater,but after long-term operation,there are problems such as adhering to the wall,water discharge safety,and poor stability.Therefore,based on Aspergillus sp.XJ-2,C.sorokiniana XJK,this article is used to remove reactive brilliant blue X-BR dye from water.By adding chitosan,a microbial algal chitosan system is constructed to enhance the stability of the microbial algal system,improve the effluent quality,and promote the effective operation of the photobioreactor.Construction of a bacterial algal chitosan composite system:By optimizing the addition sequence and amount,a bacterial algal chitosan composite system was constructed,with a flocculation efficiency of99.58%for chlorella vulgaris and a decolorization rate of 98.81%for reactive brilliant blue X-BR dye within24 hours.XPS and FITR characterization of the bacterial algal chitosan composite system showed that C-C and C-O were significantly enhanced.EEM detection of the supernatant showed the presence of excellent amino acids,and the flocculation of bacteria and algae may be positively correlated with the relative content of these groups and amino acids.The Stability of Bacterial Algae Chitosan Composite System:Under the conditions of light intensity of 3300Lux and aeration rate of 1 L·min^-1,100 mg·L^-1 reactive blue X-BR dye was treated.The stability of the bacterial algal chitosan composite system was studied by adding 100 mg·L^-1 of dye every 96 hours.The dye removal rate of the bacterial algal chitosan system after 6 hours was 93.8%.In the third and fourth cycles,the decolorization rate decreased to 22.6%.COD,T-P,and NH₄⁺-N in water can be effectively removed.The effect of different aeration rates on the stability of the bacterial algal chitosan system was investigated.The results showed that even under a high aeration rate of 3 L·min^-1,the removal rate of the bacterial algal chitosan composite system for reactive brilliant blue X-BR was above 95%.After adding chitosan,there was no adhesion phenomenon.Optimization of a columnar photobioreactor:Set a built-in light source,adjust the aeration method,increase the diameter to height ratio to 1:2,under lighting conditions of D:L=12 h:12 h,and the working volume to 1 L.In a columnar photobioreactor,the removal rate of reactive brilliant blue X-BR dye by the bacterial algal chitosan system was above 99.8%within 12 hours.When the reactor was scaled up to 5 L,the removal rate of reactive brilliant blue X-BR reached above 95%within 12 hours.In this paper,by adding chitosan,the flocculation efficiency of Chlorella was improved,and the bacteria-chitosan system was more stable in the decolorization process of active brilliant blue X-BR dye.It lays a foundation for the application of fungus-chitosan system in wastewater treatment.