Preparation And Properties Of Cu-Ag Bimetallic Nanoparticle

Microorganisms exist widely in human body and nature,and their uncontrolled rapid propagation will cause a series of problems.Overuse of traditional antimicrobials tends to lead to increased microbial resistance.At the same time,resistance reduces the efficiency of antibiotics.With the continuous development of nanoscience,nanomaterials have become promising antibacterial materials due to their unique properties and broad spectrum antibacterial activities.Cu-Ag bimetallic nanoparticles have strong antibacterial activity,which has been widely studied for its advantages of high biosafety,antibacterial efficiency and broad-spectrum antibacterial activity.In this paper,Cu-Ag bimetallic nanoparticles were prepared by chemical reduction method using glycol as solvent,Cu SO₄·5H₂O as soluble copper salt,Ag NO₃ as soluble silver salt,sodium hypophosphatite and sodium citrate as reducing agents.Different dispersants have great influence on the system of Cu-Ag bimetallic nanoparticles due to their own properties and different interaction forces with nanoparticles.XRD results show that impurity peaks appear in Cu-Ag bimetallic nanoparticles prepared without dispersant,and TEM directly reflects that the agglomeration of nanoparticles is more serious.The dispersion of Cu-Ag/PVP nanoparticles was the best after the addition of monodispersant.Based on this,the effects of PVP&SDS and PVP&CTAB on the morphology of Cu-Ag nanoparticles were investigated based on PVP.The results show that the Cu-Ag/PVP&CTAB system has the best dispersion and stability.In this paper,molecular dynamics was used to simulate Cu-Ag bimetallic nanoparticles in different systems to further study the internal relationship between Cu-Ag nanoparticles and dispersants.The results show that the interaction between Cu-Ag and dispersant is dominated by van der Waals force.The Cu-Ag/PVP system had lower total energy,higher interaction energy and the best dispersion.The interaction energy of Cu-Ag/PVP&CTAB system is higher and the dispersion is the best.The antibacterial activity of the prepared Cu-Ag bimetallic nanoparticles was qualitatively characterized by AGAR plate diffusion method,and then the antibacterial activity of Cu-Ag/dispersant system was studied by measuring the minimum bacteriostatic concentration and the minimum bactericidal concentration.The results showed that Cu-Ag/CTAB had the best antibacterial activity in the monodispersant system.The antibacterial activity of Cu-Ag/PVP&CTAB was the best in the double dispersant system,and the antibacterial activity was broad spectrum.According to the cytocompatibility test,when Cu-Ag/PVP is 8 ppm,Cu-Ag/SDS is 4 ppm,Cu-Ag/CTAB is 0.0625 ppm,Cu-Ag/PVP&SDS is 8 ppm,Cu-Ag/PVP&CTAB is 0.0625 ppm,nanoparticles are low or non-toxic to cells.