| Coal,the most abundant fossil fuel in the world,is used to generate heat,produce electricity,and make steel and industrial products,which produces several types of emissions that adversely affect the environment,particularly coking wastewater.White rot fungus have great potential to treat coking wastewater due to their unique ability to degrade refractory organic pollutants.However,white-rot fungal treatment system has been impeded owing to short-term fungal activities in reactor.A novel design is proposed to build a hollow tube type white rot fungus reactor for white rot fungus to achieve the continuous Landfill wastewater treatment based on the vascular carriers.But the vascular carrier was prone to deformation during the operation of hollow tube white-rot fungal reactor,which affected the growth of biofilms and the recycling of wastewater in reactor.Furthermore,it remains to be studied whether our fungal wastewater treatment system can be used to treat other wastewater except landfill leachate.In order to solve those problems,vascular carrier were ameliorated that Spiral stainless steel wire were fixed into the hollow cylinder of vascular carrier to supports it.Furthermore,a stable white-rot fungal treatment system was established and ran in repeated-batch mode to continuously treat coking wastewater in our hollow tube white-rot fungal reactor.The experimental results showed that the continuous 280-day growth of P chrysosporium aerobic filament biofilm in coking wastewater was successfully achieved in our hollow tube white-rot fungal reactor and the vascular carrier supported by Spiral stainless was not deformed during the operation of reactor.In addition,the lignin peroxidase(LiP)can be continuously synthesized to degrade coking wastewater,and its activity is maintained at 36 U/L to 206 U/L during the operation of reactor.During 11 successive batches,aerobic mycelium biofilm of P.chrysosporium maintained the continuous Li P production to achieved 19~25%COD removal rate(removed from 1 309mg·L-1to 453~1 064 mg·L-1),18~40%ammonium nitrogen removal rate(removed from 323mg·L-1to 194~265 mg·L-1),and 44~54%phosphate removal rate(removed from 4.0 mg·L-1to 1.8~2.2 mg·L-1).And the Aerobic Mycelium Biofilm(AMB)of P.chrysosporium can change the p H of coking wastewater from 8.0 to 6.1~6.4 in each batch.All these results suggested that the hollow tube white-rot fungal reactor can be used to treat coking wastewater except landfill leachate,that is,our white-rot fungal wastewater treatment system based on vascular carriers is repeatable,which is foundational not only for treating the wastewaters containing refractory organic pollutants using white-rot fungi,but also for establishing a fungal wastewater treatment technology. |