Preparation Of Copper-Based Bimetallic MOF Nanoenzyme And Dual-Mode Detections

Nanozymes can simulate natural enzyme activity to catalyze reactions.They have unique advantages such as high stability,low environmental dependence,low cost,and easy preparation.They have great application potential in the fields of biochemical sensing,environmental governance,disease diagnosis and treatment,and industrial production.So far,a variety of nanomaterials have been widely used in nanozyme design.However,the cost of noble metal-based materials is high,and the design of semiconductor nanomaterials such as metal compounds and carbon-based materials is complicated.Moreover,the preparation process of most materials is complicated,the separation is difficult,and only a single signal can be provided.Compared with traditional nano-enzyme materials,MOF has lower cost,is easier to prepare,and has better stability.The design and adjustment of its structure are simpler and more flexible,and it is convenient to endow MOF with more physical properties such as optical or electrical properties,so as to realize multifunctional sensing.Therefore,the construction of a nanozyme with simple preparation,low cost,high catalytic activity and multifunctional properties is of great significance for applied research.MOF materials are porous materials composed of metal centers and bridges of organic ligands.The uniformly distributed metal centers provide a large number of active sites.The higher porosity provides higher adsorption and larger specific surface area,which ensures that the catalytic sites are fully exposed and can achieve rapid catalysis of reacting substances.How to improve the catalytic activity and make it multifunctional is the focus of research on the application of MOF nanozymes,and the construction of MOFs with bimetallic active centers is an effective means to solve this problem.Compared with MOFs with single metal centers,bimetallic organic frameworks will take advantage of the structural complexity and synergy produced by different metal centers to show more excellent performance,providing more possibilities for the application of biochemical sensors.In this paper,based on Cu-based MOF,CuNi-MOF and CuFe-MOF binary MOF materials were synthesized respectively,and the peroxidase-like catalytic activity of MOF materials was studied,forming a highly sensitive sensing system.The specific research contents are as follows:（1）Two-dimensional lamellar Cu-MOF,Fe-MOF and CuFe-MOF were synthesized at room temperature by ultrasonic-assisted synthesis method.Using TMB as the chromogenic substrate,the color enhancement process of binary metal-centered MOF and monometallic-centered MOF was compared.It was confirmed that CuFe-MOF exhibited enhanced peroxidase-like activity and a stronger affinity for H₂O₂ due to the synergistic effect between the bimetallic centers compared to single-metal center MOFs.A colorimetric sensing system was constructed based on CuFe-MOF material,and the high-sensitivity detection of glucose was realized.（2）On the basis of the previous chapter,Ni,which has excellent activity in glucose electrochemical catalysis,was selected as the second metal center,and two-dimensional lamellar Cu-MOF,Ni-MOF and CuNi-MOF.Using TMB as a chromogenic substrate,the catalytic activities of binary metal-centered MOFs and monometallic-centered MOFs were compared.It was confirmed that CuNi-MOF exhibited enhanced peroxidase-like activity due to the synergy between the bimetallic centers compared to single metal center MOFs.Combined with material properties,a colorimetric-electrochemical sensor was constructed,and dual-mode sensitive detection of glucose was realized.