| The woody oil industry is our traditional industry.In the edible oil industry,a large amount of camellia seed meal is produced after oil extraction,and one of the main functional components is tea saponin.Due to its complex structure.the research on tea saponin mainly focuses on the extraction and separation of monomer,and the development and application of tea saponin derivatives are very few.This paper mainly focuses on the high value utilization of tea saponin.Firstly,the yield of tea aglycones was improved by alcoholysis of tea saponin with solid acid.Secondly fluorocarbon modification of tea saponin was carried out to prepare tea saponin fluorocarbon ester derivatives with different fluorocarbon contents.Finally,Pickering emulsion was prepared by using heptafluorobutanol tea-saponin(HFTS)granules.The main research contents and conclusions are as follows:(1)Using tea seed meal as the main raw material doped with different quality of acrylic acid.carbon-based solid acid catalyst was synthesized by carbonization and sulfonation,and its structure was characterized by SEM,FTIR.XPS.XRD and other means.Taking the yield of tea aglycones as index,the effects of alcoholysis temperature,alcoholysis time and the amount of solid acid added on the yield of tea aglycones were investigated by single variable method.The results showed that the best yield of tea saponin was 45.3%when the mass ratio of TSA/C-SO3H to tea saponin was 1:1 at 100℃.which was 28.8%higher than that of traditional hydrochloric acid hydrolysis..(2)Esterification of tea saponin with four kinds of fluorocarbon alcohols under the catalysis of XtalFluor-E was carried out to obtain tea saponin fluorocarbon derivatives with different fluorocarbon contents.Many micropores appeared on the surface of the derivative particles,and the contact angle were close to 90°.In addition,the cytotoxicity of heptafluorobutanol teasaponin(HFTS)was slightly higher than that of tea saponin at 10-40 μg·mL-1,but the overall change was not significant,indicating that the introduction of heptafluorobutanol(HFA)had no significant effect on the bioactivity of tea saponin.(3)Pickering emulsion stabilized by HFTS particles was prepared.The experimental results show that the emulsion has no obvious structural changes at room temperature,but the freezethaw stability needs to be improved.When the concentration of HFTS was 1.5wt%and the oilwater ratio(V:V)was 6:4,the emulsion with good stability could be formed,and the ionic strength(0-300 mM)had little effect on the emulsion.The coating rate of curcumin in HFTS emulsion reached 85.5%,and the DPPH free radical scavenging rate of curcumin coated by heat treatment and UV treatment increased by 38.9%and 23.8%,respectively. |
