Construction Of The Automatic Culture Module In Enzyme High-Throughput Screening System

Individual steps in biosynthetic experiments are complex and need to be repeated,and if a biosynthetic system can be developed that completes the experimental tasks automatically,increases experimental efficiency and also frees experimenters from these experimental steps,experimenters can devote more effort to the innovation of experimental content.In recent years,the automation techniques applied in the field of biomedicine are well developed,and many laboratories have built biosynthesis automation systems.However,the hardware and software in these systems are mostly commercially mature matching products,the price is relatively expensive,and it is more difficult to change the custom system after it is built.This paper aims to develop the hardware and software system and build a culture module for an automated system for high-throughput screening of enzymes.In this study,the purchased SCARA robotic arm,the purchased shaking incubator,as well as self-developed auxiliary devices were integrated to construct a fully functional and controlled simple culture platform,in which the auxiliary devices mainly include a rotating stack,a consumable storage device,as well as the auxiliary device of the shaking incubator consisting of a96-well plate fixed device and a transfer station.The main work of this paper is as follows:(1)Based on the program interface documentation provided by the purchased SCARA robot arm and the parameter address information provided by the purchased shaking incubator,the automatic control of the SCARA robotic arm and shaking incubator was realized based on the TCP communication protocol and the Modbus communication protocol.(2)Based on the functional requirements of the device,the structural design of the auxiliary device was completed using Creo three-dimensional modeling software,Arduino mega2560 controllers and associated sensors and driving elements were used to build the lower computer systems of the auxiliary device,and the automatic control of the auxiliary device was realized through the serial communication protocol and the specific customized communication rules.(3)In order to connect all devices,a system data platform for the cultivation module was constructed based on the SQL Server,and the HMI(human machine interface)of the cultivation module was built based on Visual Studio and the C# programming language.(4)In the module testing process,the stability of the auxiliary devices and the effect of the automatic control of the individual devices were verified according to the experimental steps set on the system software to achieve the goal of the collaborative work of the various devices in the module.The experimental results not only verify the feasibility of the module but also lay the foundation for the subsequent development of devices with other related functions.