Application Of Chitosan Composite Nanogels Based On Plant Nanocellulose In Wound Repair

Objective:Sterile gauze has traditionally been used as a medical dressing to treat skin wounds,but it has several shortcomings,including poor biocompatibility and material permeability,difficulties in degradation,and maintaining a wet wound environment.Additionally,its antibacterial effect is relatively poor,which can lead to re-infection and adverse effects on wound repair.This research explores the use of Chitosan(CS),a natural polymer material,which has hemostatic effects,good antibacterial ability,and accelerates wound repair.Polyvinyl alcohol(PVA)is an organic substance with excellent biocompatibility,and nano-crystalline cellulose(CNC)has excellent breathability and water absorption properties,making it suitable for wound repair.This study combines CS and PVA into the CNC to create a ternary composite antibacterial gel membrane,which can provide a new treatment plan for repairing large and difficult-to-healing skin wounds.Methods:The study used vitamin enzymes to prepare the CNC through enzymatic hydrolysis of artificial bamboo pulp fibers.A step-by-step aggregation method was used to graft chitosan onto polyvinyl alcohol,and aldehyde-based nano cellulose was polymerized using a rotating low-pressure method to prepare the ternary composite antibacterial gel membrane.The physical and chemical properties of the composite antibacterial gel film were evaluated using electron microscopy and Fourier infrared spectroscopy.The antibacterial ability of the gel film was tested using ultraviolet visible spectrophotometry,and cytotoxicity was evaluated using MTT.Hemolysis performance was measured using ultraviolet spectrophotometry,and the repair effect of the compound antibacterial gel film was investigated using rat wounds as the detection model.Wound repair was evaluated using H&E staining and Masson staining.All data were statistically analyzed using SPSS 26.0 statistical software,and the resulting images were edited and processed using Photoshop 2019.The results were expressed as mean±standard deviation.The microbiological test results were statistically evaluated by analysis of variance(post-hoc,Tukey’s test),and the level of statistical significance was set at P<0.05.Results:The ternary composite antibacterial gel film CS,PVA,and CNC had a regular and smooth surface,a complete spherical shape,and rich and evenly distributed microchannels.It was a stable medical material with an ideal microstructure,excellent water absorption,and breathability.It effectively inhibited the proliferation of Staphylococcus aureus and Escherichia coli and had a good biosafety profile.Additionally,it had a very obvious anticoagulant effect and could reduce the inflammatory reaction of the skin,helped wound repair,and reduced the formation of scars.Conclusion:The ternary composite antibacterial gel membranes CS,PVA,and CNC have many excellent characteristics,including easy film formation,strong permeability and water absorption,rapid hemostasis,effective prevention of infection,maintenance of moisture,sealing and protection of wounds,effective promotion of epidermal regeneration,and wound repair.Its transparent appearance makes it convenient for observing wound repair,reducing the frequency of dressing replacement,and providing a new treatment plan for the repair and treatment of large and difficult-to-healing skin wounds.